Literature summary extracted from
Sasaki, K.; Ohara, K.; Yazaki, K.
Gene expression and characterization of isoprene synthase from Populus alba (2005), FEBS Lett., 579, 2514-2518.
Activating Compound
EC Number |
Activating Compound |
Comment |
Organism |
Structure |
---|
4.2.3.27 |
additional information |
the enzyme is induced by heat stress and continuous light irradiation |
Populus alba |
|
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
4.2.3.27 |
gene ispS, DNA and amino acid sequence determination and analysis, expression in Escherichia coli, expression as GFP-fusion protein in onion peels and tobacco leaves, transformations by particle bombardement |
Populus alba |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
4.2.3.27 |
additional information |
enzyme expression is highly reduced in the dark |
Populus alba |
|
Localization
EC Number |
Localization |
Comment |
Organism |
GeneOntology No. |
Textmining |
---|
4.2.3.27 |
plastid |
the enzyme contains a plastidial targeting sequence |
Populus alba |
9536 |
- |
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
4.2.3.27 |
Co2+ |
activates, 23% of the activity with Mg2+ |
Populus alba |
|
4.2.3.27 |
Fe2+ |
2% of the activity with Mg2+ |
Populus alba |
|
4.2.3.27 |
Mg2+ |
activates at 20 mM, best divalent cation |
Populus alba |
|
4.2.3.27 |
Mn2+ |
activates, 34% of the activity with Mg2+ |
Populus alba |
|
4.2.3.27 |
additional information |
the enzyme requires divalent cations, no activity with Cu2+ and Ca2+ |
Populus alba |
|
4.2.3.27 |
Ni2+ |
2% of the activity with Mg2+ |
Populus alba |
|
4.2.3.27 |
Zn2+ |
3% of the activity with Mg2+ |
Populus alba |
|
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
4.2.3.27 |
dimethylallyl diphosphate |
Populus alba |
- |
isoprene + diphosphate |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
4.2.3.27 |
Populus alba |
Q50L36 |
gene ispS; gene ispS |
- |
Posttranslational Modification
EC Number |
Posttranslational Modification |
Comment |
Organism |
---|
4.2.3.27 |
proteolytic modification |
the enzyme contains a plastidial targeting sequence |
Populus alba |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
4.2.3.27 |
recombinant enzyme from Escherichia coli |
Populus alba |
Source Tissue
EC Number |
Source Tissue |
Comment |
Organism |
Textmining |
---|
4.2.3.27 |
cell culture |
from leaves |
Populus alba |
- |
4.2.3.27 |
leaf |
- |
Populus alba |
- |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
4.2.3.27 |
0.00039 |
- |
leaf cell culture |
Populus alba |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
4.2.3.27 |
dimethylallyl diphosphate |
- |
Populus alba |
isoprene + diphosphate |
- |
? |
|
4.2.3.27 |
dimethylallyl diphosphate |
the enzyme is absolutely specific for dimethylally diphosphate |
Populus alba |
isoprene + diphosphate |
product identification |
? |
|
4.2.3.27 |
additional information |
substrate specifcity, no enzymatic activity with isoprenyl diphosphate or geranyl diphosphate as substrates, overview |
Populus alba |
? |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
4.2.3.27 |
More |
the enzyme contains the aspartate-rich motif DDxxD |
Populus alba |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
4.2.3.27 |
ISPS |
- |
Populus alba |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
4.2.3.27 |
40 |
- |
recombinant enzyme |
Populus alba |
Temperature Range [°C]
EC Number |
Temperature Minimum [°C] |
Temperature Maximum [°C] |
Comment |
Organism |
---|
4.2.3.27 |
25 |
50 |
about half-maximal activity at 25°C and 50°C, temperature profile |
Populus alba |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
4.2.3.27 |
8 |
- |
recombinant enzyme |
Populus alba |
pH Range
EC Number |
pH Minimum |
pH Maximum |
Comment |
Organism |
---|
4.2.3.27 |
6.5 |
9.5 |
activity range |
Populus alba |