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Literature summary extracted from
- Complete reversal of coenzyme specificity of xylitol dehydrogenase and increase of thermostability by the introduction of structural zinc (2005), J. Biol. Chem., 280, 10340-10349.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.1.1.9 | expression in Escherichia coli | Scheffersomyces stipitis |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.1.1.9 | D207A | kcat/Km for NAD+ is 3.6fold lower than wild-type value, kcat/Km for NADP+ is 4.3fold higher than wild-type value | Scheffersomyces stipitis |
| 1.1.1.9 | D207A/F209S | kcat/Km for NAD+ is 2.2fold lower than wild-type value, kcat/Km for NADP+ is 745fold higher than wild-type value | Scheffersomyces stipitis |
| 1.1.1.9 | D207A/I208R | kcat/Km for NAD+ is 2.5fold lower than wild-type value, kcat/Km for NADP+ is 229fold higher than wild-type value | Scheffersomyces stipitis |
| 1.1.1.9 | D207A/I208R/F209S | kcat/Km for NAD+ is 15.2fold lower than wild-type value, kcat/Km for NADP+ is 4292fold higher than wild-type value, increased thermostability | Scheffersomyces stipitis |
| 1.1.1.9 | D207A/I208R/F209S/N211R | kcat/Km for NAD+ is 32.9fold lower than wild-type value, kcat/Km for NADP+ is 4292fold higher than wild-type value, increased thermostability | Scheffersomyces stipitis |
| 1.1.1.9 | D207A/I208R/F209T | kcat/Km for NAD+ is 2.4fold lower than wild-type value, kcat/Km for NADP+ is 4754fold higher than wild-type value | Scheffersomyces stipitis |
| 1.1.1.9 | D207A/I208R/F209Y | kcat/Km for NAD+ is 6.9fold lowerthan wild-type value, kcat/Km for NADP+ is 788fold higher than wild-type value | Scheffersomyces stipitis |
| 1.1.1.9 | F209S | kcat/Km for NAD+ is 1.9fold lower than wild-type value, kcat/Km for NADP+ is 31.4fold higher than wild-type value | Scheffersomyces stipitis |
| 1.1.1.9 | I208R | kcat/Km for NAD+ is nearly identical to wild-type value, kcat/Km for NADP+ is 44fold higher than wild-type value | Scheffersomyces stipitis |
| 1.1.1.9 | I208R/F209S | kcat/Km for NAD+ is 30.7fold lower than wild-type value, kcat/Km for NADP+ is 1.5fold higher than wild-type value | Scheffersomyces stipitis |
| 1.1.1.9 | N211R | kcat/Km for NAD+ is 1.1fold lower than wild-type value, kcat/Km for NADP+ is 7.6fold higher than wild-type value | Scheffersomyces stipitis |
| 1.1.1.9 | S96C/S99C/Y102C/D207A/I208R/F209S | kcat/Km for NAD+ is 36.7fold lower than wild-type value, kcat/Km for NADP+ 16462is fold higher than wild-type value | Scheffersomyces stipitis |
| 1.1.1.9 | S96C/S99C/Y102C/D207A/I208R/F209S/N211R | kcat/Km for NAD+ is 26.5fold lower than wild-type value, kcat/Km for NADP+ is 16154fold higher than wild-type value | Scheffersomyces stipitis |
| 1.1.1.9 | S96C/S99CY102C | kcat/Km for NAD+ is 1.1fold lower than wild-type value, kcat/Km for NADP+ is 8.8fold higher than wild-type value | Scheffersomyces stipitis |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.9 | 0.152 | - |
NAD+ | pH 9.0, 35°C, mutant enzyme D207A/I208R/F209Y | Scheffersomyces stipitis |  |
| 1.1.1.9 | 0.162 | - |
NAD+ | pH 9.0, 35°C, mutant enzyme D207A/F209S | Scheffersomyces stipitis | |
| 1.1.1.9 | 0.265 | - |
NAD+ | pH 9.0, 35°C, mutant enzyme D207A/I208R/F209T | Scheffersomyces stipitis | |
| 1.1.1.9 | 0.381 | - |
NAD+ | pH 9.0, 35°, wild-type enzyme | Scheffersomyces stipitis | |
| 1.1.1.9 | 0.403 | - |
NAD+ | pH 9.0, 35°C, mutant enzyme D207A | Scheffersomyces stipitis | |
| 1.1.1.9 | 0.498 | - |
NAD+ | pH 9.0, 35°C, mutant enzyme I208R | Scheffersomyces stipitis | |
| 1.1.1.9 | 0.538 | - |
NAD+ | pH 9.0, 35°C, mutant enzyme N211R | Scheffersomyces stipitis | |
| 1.1.1.9 | 0.568 | - |
NAD+ | pH 9.0, 35°C, mutant enzyme D207A/I208R | Scheffersomyces stipitis | |
| 1.1.1.9 | 0.638 | - |
xylitol | pH 9.0, 35°C, cofactor: NADP+, mutant enzyme D207A/I208R/F209T | Scheffersomyces stipitis | |
| 1.1.1.9 | 0.638 | - |
NADP+ | pH 9.0, 35°C, mutant enzyme D207A/I208R/F209T | Scheffersomyces stipitis | |
| 1.1.1.9 | 0.665 | - |
NAD+ | pH 9.0, 35°C, mutant enzyme I208R/F209S | Scheffersomyces stipitis | |
| 1.1.1.9 | 0.731 | - |
xylitol | pH 9.0, 35°C, cofactor: NADP+, mutant enzyme D207A/I208R/F209Y | Scheffersomyces stipitis | |
| 1.1.1.9 | 0.731 | - |
NADP+ | pH 9.0, 35°C, mutant enzyme D207A/I208R/F209Y | Scheffersomyces stipitis | |
| 1.1.1.9 | 0.739 | - |
NAD+ | pH 9.0, 35°C, mutant enzyme S96C/S99CY102C | Scheffersomyces stipitis | |
| 1.1.1.9 | 0.848 | - |
NAD+ | pH 9.0, 35°C, mutant enzyme F209S | Scheffersomyces stipitis | |
| 1.1.1.9 | 0.897 | - |
xylitol | pH 9.0, 35°C, cofactor: NADP+, mutant enzyme D207A/I208R/F209S | Scheffersomyces stipitis | |
| 1.1.1.9 | 0.897 | - |
NADP+ | pH 9.0, 35°C, mutant enzyme D207A/I208R/F209S | Scheffersomyces stipitis | |
| 1.1.1.9 | 1.04 | - |
xylitol | pH 9.0, 35°C, cofactor: NADP+, mutant enzyme S96C/S99C/Y102C/D207A/I208R/F209S/N211R | Scheffersomyces stipitis | |
| 1.1.1.9 | 1.04 | - |
NADP+ | pH 9.0, 35°C, mutant enzym S96C/S99C/Y102C/D207A/I208R/F209S/N211R | Scheffersomyces stipitis | |
| 1.1.1.9 | 1.18 | - |
xylitol | pH 9.0, 35°C, cofactor: NADP+, mutant enzyme S96C/S99C/Y102C/D207A/I208R/F209S | Scheffersomyces stipitis | |
| 1.1.1.9 | 1.18 | - |
NADP+ | pH 9.0, 35°C, mutant enzyme S96C/S99C/Y102C/D207A/I208R/F209S | Scheffersomyces stipitis | |
| 1.1.1.9 | 1.3 | - |
NAD+ | pH 9.0, 35°C, mutant enzyme D207A/I208R/F209S | Scheffersomyces stipitis | |
| 1.1.1.9 | 1.38 | - |
xylitol | pH 9.0, 35°C, cofactor: NADP+, mutant enzyme D207A/I208R/F209S/N211R | Scheffersomyces stipitis | |
| 1.1.1.9 | 1.38 | - |
NADP+ | pH 9.0, 35°C, mutant enzyme D207A/I208R/F209S/N211R | Scheffersomyces stipitis | |
| 1.1.1.9 | 4 | 5.4 | xylitol | pH 9.0, 35°C, cofactor: NAD+, mutant enzyme I208R/F209S | Scheffersomyces stipitis | |
| 1.1.1.9 | 7.6 | - |
NAD+ | pH 9.0, 35°C, mutant enzym S96C/S99C/Y102C/D207A/I208R/F209S/N211R | Scheffersomyces stipitis | |
| 1.1.1.9 | 9.19 | - |
NADP+ | pH 9.0, 35°C, mutant enzyme I208R/F209S | Scheffersomyces stipitis | |
| 1.1.1.9 | 9.56 | - |
xylitol | pH 9.0, 35°C, cofactor: NADP+, mutant enzyme S96C/S99CY102C | Scheffersomyces stipitis | |
| 1.1.1.9 | 9.56 | - |
NADP+ | pH 9.0, 35°C, mutant enzyme S96C/S99CY102C | Scheffersomyces stipitis | |
| 1.1.1.9 | 9.96 | - |
xylitol | pH 9.0, 35°C, cofactor: NADP+, mutant enzyme D207A/F209S | Scheffersomyces stipitis | |
| 1.1.1.9 | 9.96 | - |
NADP+ | pH 9.0, 35°C, mutant enzyme D207A/F209S | Scheffersomyces stipitis | |
| 1.1.1.9 | 11.3 | - |
NADP+ | pH 9.0, 35°C, mutant enzyme D207A/I208R | Scheffersomyces stipitis | |
| 1.1.1.9 | 17.3 | - |
NAD+ | pH 9.0, 35°C, mutant enzyme D207A/I208R/F209S/N211R | Scheffersomyces stipitis | |
| 1.1.1.9 | 21.1 | - |
NADP+ | pH 9.0, 35°C, mutant enzyme I208R | Scheffersomyces stipitis | |
| 1.1.1.9 | 21.7 | - |
xylitol | pH 9.0, 35°, cofactor: NAD+, wild-type enzyme | Scheffersomyces stipitis | |
| 1.1.1.9 | 22.2 | - |
xylitol | pH 9.0, 35°C, cofactor: NAD+, mutant enzyme D207A | Scheffersomyces stipitis | |
| 1.1.1.9 | 23.5 | - |
NAD+ | pH 9.0, 35°C, mutant enzyme S96C/S99C/Y102C/D207A/I208R/F209S | Scheffersomyces stipitis | |
| 1.1.1.9 | 24.2 | - |
xylitol | pH 9.0, 35°C, cofactor: NAD+, mutant enzyme D207A/I208R | Scheffersomyces stipitis | |
| 1.1.1.9 | 27.4 | - |
xylitol | pH 9.0, 35°C, cofactor: NAD+, mutant enzyme N211R | Scheffersomyces stipitis | |
| 1.1.1.9 | 28.9 | - |
NADP+ | pH 9.0, 35°C, mutant enzyme F209S | Scheffersomyces stipitis | |
| 1.1.1.9 | 29.5 | - |
xylitol | pH 9.0, 35°C, cofactor: NAD+, mutant enzyme I208R | Scheffersomyces stipitis | |
| 1.1.1.9 | 30.3 | - |
xylitol | pH 9.0, 35°C, cofactor: NAD+, mutant enzyme S96C/S99CY102C | Scheffersomyces stipitis | |
| 1.1.1.9 | 34.1 | - |
xylitol | pH 9.0, 35°C, cofactor: NAD+, mutant enzyme F209S | Scheffersomyces stipitis | |
| 1.1.1.9 | 45.2 | - |
xylitol | pH 9.0, 35°C, cofactor: NAD+, mutant enzyme D207A/F209S | Scheffersomyces stipitis | |
| 1.1.1.9 | 50.1 | - |
xylitol | pH 9.0, 35°C, cofactor: NAD+, mutant enzyme D207A/I208R/F209Y | Scheffersomyces stipitis | |
| 1.1.1.9 | 55.7 | - |
xylitol | pH 9.0, 35°C, cofactor: NAD+, mutant enzyme D207A/I208R/F209S | Scheffersomyces stipitis | |
| 1.1.1.9 | 56.5 | - |
NADP+ | pH 9.0, 35°C, mutant enzyme N211R | Scheffersomyces stipitis | |
| 1.1.1.9 | 97.8 | - |
xylitol | pH 9.0, 35°C, cofactor: NAD+, mutant enzyme D207A/I208R/F209T | Scheffersomyces stipitis | |
| 1.1.1.9 | 120 | - |
NADP+ | pH 9.0, 35°C, mutant enzyme D207A | Scheffersomyces stipitis | |
| 1.1.1.9 | 170 | - |
NADP+ | pH 9.0, 35°, wild-type enzyme | Scheffersomyces stipitis | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.9 | Scheffersomyces stipitis | P22144 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.9 | recombinant enzyme | Scheffersomyces stipitis |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.9 | additional information | - |
specific activity of wild-type and mutant enzymes | Scheffersomyces stipitis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.9 | xylitol + NAD+ | - |
Scheffersomyces stipitis | D-xylulose + NADH + H+ | - |
? | |
| 1.1.1.9 | xylitol + NADP+ | - |
Scheffersomyces stipitis | D-xylulose + NADPH + H+ | - |
? | |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.9 | 0.017 | - |
NADP+ | pH 9.0, 35°C, mutant enzyme D207A/I208R/F209T | Scheffersomyces stipitis | |
| 1.1.1.9 | 0.052 | 2.1 | NAD+ | pH 9.0, 35°C, mutant enzyme D207A/I208R | Scheffersomyces stipitis | |
| 1.1.1.9 | 0.145 | - |
NADP+ | pH 9.0, 35°C, mutant enzyme I208R/F209S | Scheffersomyces stipitis | |
| 1.1.1.9 | 0.5 | - |
NAD+ | pH 9.0, 35°C, mutant enzym S96C/S99C/Y102C/D207A/I208R/F209S/N211R | Scheffersomyces stipitis | |
| 1.1.1.9 | 0.91 | - |
NADP+ | pH 9.0, 35°C, mutant enzyme S96C/S99CY102C | Scheffersomyces stipitis | |
| 1.1.1.9 | 1 | - |
NAD+ | pH 9.0, 35°C, mutant enzyme D207A/I208R/F209Y | Scheffersomyces stipitis | |
| 1.1.1.9 | 1 | - |
NAD+ | pH 9.0, 35°C, mutant enzyme I208R/F209S | Scheffersomyces stipitis | |
| 1.1.1.9 | 1.83 | - |
NADP+ | pH 9.0, 35°, wild-type enzyme | Scheffersomyces stipitis | |
| 1.1.1.9 | 2 | 8 | NADP+ | pH 9.0, 35°C, mutant enzyme D207A/I208R | Scheffersomyces stipitis | |
| 1.1.1.9 | 3.33 | - |
NAD+ | pH 9.0, 35°C, mutant enzyme D207A/F209S | Scheffersomyces stipitis | |
| 1.1.1.9 | 4 | - |
NAD+ | pH 9.0, 35°C, mutant enzyme D207A/I208R/F209S | Scheffersomyces stipitis | |
| 1.1.1.9 | 4.67 | - |
NADP+ | pH 9.0, 35°C, mutant enzyme N211R | Scheffersomyces stipitis | |
| 1.1.1.9 | 5.17 | - |
NAD+ | pH 9.0, 35°C, mutant enzyme D207A | Scheffersomyces stipitis | |
| 1.1.1.9 | 5.17 | - |
NAD+ | pH 9.0, 35°C, mutant enzyme D207A/I208R/F209T | Scheffersomyces stipitis | |
| 1.1.1.9 | 5.7 | - |
NADP+ | pH 9.0, 35°C, mutant enzyme D207A | Scheffersomyces stipitis | |
| 1.1.1.9 | 6.17 | - |
NADP+ | pH 9.0, 35°C, mutant enzyme D207A/I208R/F209Y | Scheffersomyces stipitis | |
| 1.1.1.9 | 8 | - |
NADP+ | pH 9.0, 35°C, mutant enzyme D207A/F209S | Scheffersomyces stipitis | |
| 1.1.1.9 | 9.83 | - |
NADP+ | pH 9.0, 35°C, mutant enzyme F209S | Scheffersomyces stipitis | |
| 1.1.1.9 | 10 | - |
NADP+ | pH 9.0, 35°C, mutant enzyme I208R | Scheffersomyces stipitis | |
| 1.1.1.9 | 10.33 | - |
NAD+ | pH 9.0, 35°C, mutant enzyme D207A/I208R | Scheffersomyces stipitis | |
| 1.1.1.9 | 13.17 | - |
NAD+ | pH 9.0, 35°C, mutant enzym S96C/S99C/Y102C/D207A/I208R/F209S/N211R | Scheffersomyces stipitis | |
| 1.1.1.9 | 16.9 | - |
NAD+ | pH 9.0, 35°C, mutant enzyme N211R | Scheffersomyces stipitis | |
| 1.1.1.9 | 17.5 | - |
NAD+ | pH 9.0, 35°, wild-type enzyme | Scheffersomyces stipitis | |
| 1.1.1.9 | 18 | - |
NAD+ | pH 9.0, 35°C, mutant enzyme D207A/I208R/F209S/N211R | Scheffersomyces stipitis | |
| 1.1.1.9 | 20.33 | - |
NAD+ | pH 9.0, 35°C, mutant enzyme F209S | Scheffersomyces stipitis | |
| 1.1.1.9 | 22.83 | - |
NAD+ | pH 9.0, 35°C, mutant enzyme N211R | Scheffersomyces stipitis | |
| 1.1.1.9 | 23.8 | - |
NAD+ | pH 9.0, 35°C, mutant enzyme I208R | Scheffersomyces stipitis | |
| 1.1.1.9 | 23.83 | - |
NAD+ | pH 9.0, 35°C, mutant enzyme D207A/I208R/F209S/N211R | Scheffersomyces stipitis | |
| 1.1.1.9 | 29.5 | - |
NAD+ | pH 9.0, 35°C, mutant enzyme S96C/S99C/Y102C/D207A/I208R/F209S | Scheffersomyces stipitis | |
| 1.1.1.9 | 30.33 | - |
NAD+ | pH 9.0, 35°C, mutant enzyme S96C/S99CY102C | Scheffersomyces stipitis | |
| 1.1.1.9 | 32.83 | - |
NADP+ | pH 9.0, 35°C, mutant enzyme D207A/I208R/F209T | Scheffersomyces stipitis | |
| 1.1.1.9 | 41.7 | - |
NADP+ | pH 9.0, 35°C, mutant enzyme D207A/I208R/F209S | Scheffersomyces stipitis | |
| 1.1.1.9 | 64 | - |
NADP+ | pH 9.0, 35°C, mutant enzyme D207A/I208R/F209S/N211R | Scheffersomyces stipitis | |
| 1.1.1.9 | 183.3 | - |
NADP+ | pH 9.0, 35°C, mutant enzym S96C/S99C/Y102C/D207A/I208R/F209S/N211R | Scheffersomyces stipitis | |
| 1.1.1.9 | 210 | - |
NADP+ | pH 9.0, 35°C, mutant enzyme S96C/S99C/Y102C/D207A/I208R/F209S | Scheffersomyces stipitis | |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.9 | NAD+ | kcat/Km for wild-type enzyme is 2760/min*mM | Scheffersomyces stipitis | |
| 1.1.1.9 | NADP+ | kcat/Km for wild-type enzyme is 0.65/min*mM | Scheffersomyces stipitis | |
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