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Literature summary extracted from

  • Phartiyal, P.; Kim, W.S.; Cahoon, R.E.; Jez, J.M.; Krishnan, H.B.
    Soybean ATP sulfurylase, a homodimeric enzyme involved in sulfur assimilation, is abundantly expressed in roots and induced by cold treatment (2006), Arch. Biochem. Biophys., 450, 20-29.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.7.7.4 agriculture increasing the expression level of this key enzyme during soybean seed development could lead to an increase in the availability of sulfur amino acids, thereby enhancing the nutritional value of the crop Glycine max

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.4 cloned in Escherichia coli as a truncated version of soybean ATPS lacking the first 48 amino acids, removal of the putative localisation sequence improves the yield of N-terminally His-tagged protein in Escherichia coli Glycine max
2.7.7.4 the protein is insoluble when the full-length cDNA of soybean ATPS, including the chloroplast/plastid localisation sequence, is used for expression in Escherichia coli Glycine max

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.4 DELTA48 a truncated version of soybean ATPS lacking the first 48 amino acids is generated for protein expression and purification, removal of the putative localisation sequence improves the yield of N-terminally His-tagged protein in Escherichia coli Glycine max
2.7.7.4 additional information the plant enzymes only contain ATP sulfurylase domain, unlike the human and yeast enzymes which include an APS kinase domain, located at the N- or C-terminal regions, respectively Glycine max

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.4 0.00211
-
 sulfate forward reaction, APS synthesis Glycine max
2.7.7.4 0.0191
-
 diphosphate reverse reaction, ATP synthesis Glycine max
2.7.7.4 0.0191
-
 diphosphate diphosphate in form of magnesium diphosphate Glycine max
2.7.7.4 0.0245
-
 adenylyl sulfate reverse reaction, ATP synthesis Glycine max
2.7.7.4 0.195
-
 ATP forward reaction, APS synthesis Glycine max

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.4 Mg2+ for APS synthesis assay contains 15 mM MgCl2, for ATP synthesis assay contains 5 mM MgCl2 Glycine max

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.4 additional information
-
 ATPS contains a chloroplast/plastid transit peptide sequence in its N-terminal region, processing of the transit peptide releases a mature protein of 46.4 kDa an a pI of 6.26 Glycine max
2.7.7.4 50000
-
 enzyme functions as a 2 * 50000 Da homodimer Glycine max
2.7.7.4 52000
-
 predicted from cDNA sequence, enzyme functions as a 100 kDa homodimer Glycine max

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.4 Glycine max Q8SAG1
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.7.4 additional information ATPS contains a chloroplast/plastid transit peptide sequence in its N-terminal region, processing of the transit peptide releases a mature protein of 46.4 kDa an a pI of 6.26 Glycine max

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.4 N-terminal His-tagged protein is passed over a Ni2+-NTA column Glycine max

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.4 leaf
-
 Glycine max
-
2.7.7.4 additional information by northern blot analysis a decline in the ATPS transcript level during seed development can be found Glycine max
-
2.7.7.4 root ATPS mRNA is most abundant in root tissue, cold treatment induces RNA accumulation and enhances the specific activity of ATPS in root tissue Glycine max
-
2.7.7.4 seed
-
 Glycine max
-
2.7.7.4 stem
-
 Glycine max
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.4 additional information
-
 ATPS activity gradually increases during cold treatment, after 72h at 10°C ATPS activity is 2.2fold higher Glycine max
2.7.7.4 additional information
-
 ATPS activity in stem and leaf tissue are comparable but lower than in seeds and roots Glycine max
2.7.7.4 additional information
-
 ATPS activity in young seeds is several-folds higher than in any other tissue Glycine max
2.7.7.4 2.9
-
 specific activity for APS synthesis Glycine max
2.7.7.4 48.7
-
 specific activity for ATP synthesis Glycine max

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.4 ATP + sulfate
-
 Glycine max diphosphate + adenylyl sulfate
-
 r
2.7.7.4 diphosphate + adenylyl sulfate
-
 Glycine max ATP + sulfate
-
 r
2.7.7.4 diphosphate + adenylyl sulfate diphosphate in form of magnesium diphosphate Glycine max ATP + sulfate
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.7.4 homodimer enzyme functions as a 2 * 50000 Da homodimer Glycine max

Synonyms

EC Number Synonyms Comment Organism
2.7.7.4 ATP sulfurylase
-
 Glycine max
2.7.7.4 ATPS
-
 Glycine max

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.7.4 1.49
-
 ATP forward reaction, APS synthesis Glycine max
2.7.7.4 2.11
-
 sulfate forward reaction, APS synthesis Glycine max
2.7.7.4 19.1
-
 diphosphate reverse reaction, ATP synthesis Glycine max
2.7.7.4 24.5
-
 adenylyl sulfate reverse reaction, ATP synthesis Glycine max

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.4 8
-
 assay at Glycine max

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.7.4 Glycine max ATPS contains a chloroplast/plastid transit peptide sequence in its N-terminal region, processing of the transit peptide releases a mature protein of 46.4 kDa an a pI of 6.26
-
 additional information
2.7.7.4 Glycine max predicted from cDNA sequence
-
 7.3