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Literature summary extracted from

  • Kanno, K.N.; Nagahisa, N.E.; Sato , S.M.; Sato, S.Y.
    Nonradioactive assay for adenosine 5'-phosphosulfate sulfotransferase using reversed-phase ion-pair high-performance liquid chromatography (1993), Biochem. Mol. Biol. Int., 29, 47-55.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.2.B1 0.0021
-
 adenosine 5'-phosphosulfate pH 9.5, 25°C Neopyropia yezoensis
2.8.2.B1 1.5
-
 dithiothreitol pH 9.5, 25°C Neopyropia yezoensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.2.B1 adenosine 5'-phosphosulfate + dithiothreitol Neopyropia yezoensis first reaction of the adenosine 5'-phosphosulfate pathway AMP + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.B1 Neopyropia yezoensis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.2.B1
-
 Neopyropia yezoensis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.8.2.B1 thallus
-
 Neopyropia yezoensis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.2.B1 additional information
-
 nonradioactive assay for adenosine 5-phosphosulfate sulfotransferase and its standardization using reversed-phase ion-pair high-performance liquid chromatography Neopyropia yezoensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.B1 adenosine 5'-phosphosulfate + dithioerythritol
-
 Neopyropia yezoensis AMP + ?
-
 ?
2.8.2.B1 adenosine 5'-phosphosulfate + dithiothreitol
-
 Neopyropia yezoensis AMP + ?
-
 ?
2.8.2.B1 adenosine 5'-phosphosulfate + dithiothreitol first reaction of the adenosine 5'-phosphosulfate pathway Neopyropia yezoensis AMP + ?
-
 ?
2.8.2.B1 adenosine 5'-phosphosulfate + reduced glutathione
-
 Neopyropia yezoensis AMP + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.8.2.B1 APSSTase
-
 Neopyropia yezoensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.8.2.B1 25
-
 pH 9.5, 10 min, 50% loss of activity, thermal inactivation is protected by the high concentration of Na2SO4 and to some extent by adenosine 5'-phosphosulfate Neopyropia yezoensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.2.B1 9.5
-
-
 Neopyropia yezoensis