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Literature summary extracted from

  • Zhao, Y.; Zhao, L.; Yang, G.; Tao, J.; Bu, Y.; Liao, F.
    Characterization of an uricase from Bacillus fastidious A.T.C.C. 26904 and its application to serum uric acid assay by a patented kinetic uricase method (2006), Biotechnol. Appl. Biochem., 45, 75-80.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.3.3 xanthine
-
 Metabacillus fastidiosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.3.3 0.204
-
 Urate at pH 9.2 Metabacillus fastidiosus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.3.3 36000
-
 4 * 36000, SDS-PAGE and matrix-assisted laser-desorption ionization-time-of-flight mass spectrometry Metabacillus fastidiosus
1.7.3.3 151000
-
 Sephadex G-200 gel filtration Metabacillus fastidiosus

Organism

EC Number Organism UniProt Comment Textmining
1.7.3.3 Metabacillus fastidiosus
-
 strain ATCC 26904
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Storage Stability

EC Number Storage Stability Organism
1.7.3.3 -12°C, 0.10 mol/l Tris/HCl buffer (pH 8.0), 8 months, less than 20% loss of activity, enzyme remains stable Metabacillus fastidiosus
1.7.3.3 4°C, 0.10 mol/l Tris/HCl buffer (pH 8.0), 10 weeks, less than 20% loss of activity Metabacillus fastidiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.3.3 urate + O2 + H2O
-
 Metabacillus fastidiosus 5-hydroxyisourate + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.7.3.3 tetramer 4 * 36000, SDS-PAGE and matrix-assisted laser-desorption ionization-time-of-flight mass spectrometry Metabacillus fastidiosus

Synonyms

EC Number Synonyms Comment Organism
1.7.3.3 uricase
-
 Metabacillus fastidiosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.3.3 9 10.5
-
 Metabacillus fastidiosus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.7.3.3 9 10.5
-
 Metabacillus fastidiosus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.7.3.3 0.041
-
 xanthine more than 0.03 mM Metabacillus fastidiosus