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Literature summary extracted from

  • Walraven, J.M.; Trent, J.O.; Hein, D.W.
    Computational and eperimental aalyses of mammalian arylamine N-acetyltransferase structure and function (2007), Drug Metab. Dispos., 35, 1001-1007.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.5 into the pKK223-3 bacterial expression vector for transformation of Escherichia coli JM105 cells Homo sapiens
2.3.1.5 isozyme NAT2, DNA and amino acid sequence determination and analysis, expression of wild-type and mutants in Escherichia coli strain JM105 Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.5 additional information construction of several insertion and deletion mutants of NAT2, overview Homo sapiens
2.3.1.5 additional information ANDH, human NAT2 mutant, a 17-residue segment is deleted and replaced Homo sapiens
2.3.1.5 additional information GG, human NAT2 mutant, a 17-residue segment is deleted and replaced Homo sapiens
2.3.1.5 additional information GGSG, human NAT2 mutant, a 17-residue segment is deleted and replaced Homo sapiens
2.3.1.5 additional information GRSG, human NAT2 mutant, a 17-residue segment is deleted and replaced Homo sapiens
2.3.1.5 additional information HHEH, human NAT2 mutant, a 17-residue segment is deleted and replaced Homo sapiens
2.3.1.5 additional information PHAG, human NAT2 mutant, a 17-residue segment is deleted and replaced Homo sapiens
2.3.1.5 additional information QEGST, human NAT2 mutant, a 17-residue segment is deleted and replaced Homo sapiens
2.3.1.5 additional information SDGSD, human NAT2 mutant, a 17-residue segment is deleted and replaced Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.5 acetyl-CoA + an arylamine Homo sapiens the enzyme is important for the activation and deactivation of exocyclic amine-containing pro-carcinogens, and for the metabolism of some pharmaceutical drugs CoA + an N-acetylarylamine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.5 Homo sapiens
-
-
-
2.3.1.5 Homo sapiens P11245 isozyme NAT2; isozyme NAT2
-
2.3.1.5 Homo sapiens P18440 isozyme NAT1; isozyme NAT1
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.5 acetyl-CoA + an arylamine = CoA + an N-acetylarylamine structure-function analysis, Cys68-His107-Asp122 catalytic triad Homo sapiens
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.5 additional information wide tissue distribution Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.5 0.0005
-
 mutant GGSG Homo sapiens
2.3.1.5 0.0005
-
 mutant HHEH Homo sapiens
2.3.1.5 0.0006
-
 mutant GGSG Homo sapiens
2.3.1.5 0.0006
-
 mutant HHEH Homo sapiens
2.3.1.5 0.0013
-
 mutant GRSG Homo sapiens
2.3.1.5 0.0014
-
 mutant GRSG Homo sapiens
2.3.1.5 0.0055
-
 mutant GG Homo sapiens
2.3.1.5 0.006
-
 mutant GG Homo sapiens
2.3.1.5 0.0061
-
-
 Homo sapiens
2.3.1.5 0.0064
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.5 acetyl-CoA + an arylamine the enzyme is important for the activation and deactivation of exocyclic amine-containing pro-carcinogens, and for the metabolism of some pharmaceutical drugs Homo sapiens CoA + an N-acetylarylamine
-
 ?
2.3.1.5 acetyl-CoA + an arylamine the enzyme catalyzes the N-acetylation of arylamines and hydrazines and O-acetylation of N-hydroxy-arylamines and heterocyclic amines Homo sapiens CoA + an N-acetylarylamine
-
 ?
2.3.1.5 acetyl-CoA + sulfamethazine
-
 Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.5 More secondary structures of wild-type and mutant enzymes, structure-function analysis, computational docking of NAT substrates, NAT homology modeling based on crystal structures of bacteria with Protein Data Bank accession numbers 1E2T, 1W4T, 1GX3, and 2BSZ, overview Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.3.1.5 arylamine N-acetyltransferase
-
 Homo sapiens
2.3.1.5 NAT
-
 Homo sapiens
2.3.1.5 NAT2
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.5 37
-
 assay at Homo sapiens
2.3.1.5 37
-
 activity assay Homo sapiens

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.3.1.5 30 50 control Homo sapiens
2.3.1.5 30 33 mutant GG Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.5 acetyl-CoA
-
 Homo sapiens