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Literature summary extracted from
- A new sulfurtransferase from the hyperthermophilic bacterium Aquifex aeolicus. Being single is not so simple when temperature gets high (2007), FEBS J., 274, 4572-4587.
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 2.8.1.1 | homology modeling of structure. The potential active site is located between a central beta strand and an alpha helix. Residues R30, E34 are involved in substrate binding | Aquifex aeolicus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 2.8.1.1 | 0.05 | - |
Polysulfide | pH 9.0, 80°C | Aquifex aeolicus | |
| 2.8.1.1 | 2.1 | - |
cyanide | pH 9.0, 80°C | Aquifex aeolicus |  |
| 2.8.1.1 | 5.7 | - |
thiosulfate | pH 9.0, 80°C | Aquifex aeolicus | |
| 2.8.1.1 | 6.9 | - |
tetrathionate | pH 9.0, 80°C | Aquifex aeolicus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 2.8.1.1 | periplasm | - |
Aquifex aeolicus | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 2.8.1.1 | 12800 | - |
1 * 12804, calculated, 1 * 12800, mass spectrometry, enzyme is in equilibrium between monomer, dimer and tetramer with shift to the tetrameric state in presence of substrate | Aquifex aeolicus |
| 2.8.1.1 | 12800 | - |
2 * 12804, calculated, 2 * 12800, mass spectrometry, enzyme is in equilibrium between monomer, dimer and tetramer with shift to the tetrameric state in presence of substrate | Aquifex aeolicus |
| 2.8.1.1 | 12800 | - |
4 * 12804, calculated, 4 * 12800, mass spectrometry, enzyme is in equilibrium between monomer, dimer and tetramer with shift to the tetrameric state in presence of substrate | Aquifex aeolicus |
| 2.8.1.1 | 12804 | - |
1 * 12804, calculated, 1 * 12800, mass spectrometry, enzyme is in equilibrium between monomer, dimer and tetramer with shift to the tetrameric state in presence of substrate | Aquifex aeolicus |
| 2.8.1.1 | 12804 | - |
2 * 12804, calculated, 2 * 12800, mass spectrometry, enzyme is in equilibrium between monomer, dimer and tetramer with shift to the tetrameric state in presence of substrate | Aquifex aeolicus |
| 2.8.1.1 | 12804 | - |
4 * 12804, calculated, 4 * 12800, mass spectrometry, enzyme is in equilibrium between monomer, dimer and tetramer with shift to the tetrameric state in presence of substrate | Aquifex aeolicus |
| 2.8.1.1 | 15000 | 50000 | - |
Aquifex aeolicus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.8.1.1 | Aquifex aeolicus | - |
- |
- |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 2.8.1.1 | 850 | - |
pH 9.0, 80°C | Aquifex aeolicus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.8.1.1 | polysulfide + cyanide | - |
Aquifex aeolicus | ? + thiocyanate | - |
? | |
| 2.8.1.1 | tetrathionate + cyanide | - |
Aquifex aeolicus | ? + thiocyanate | - |
? | |
| 2.8.1.1 | thiosulfate + cyanide | - |
Aquifex aeolicus | sulfite + thiocyanate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 2.8.1.1 | dimer | 2 * 12804, calculated, 2 * 12800, mass spectrometry, enzyme is in equilibrium between monomer, dimer and tetramer with shift to the tetrameric state in presence of substrate | Aquifex aeolicus |
| 2.8.1.1 | monomer | 1 * 12804, calculated, 1 * 12800, mass spectrometry, enzyme is in equilibrium between monomer, dimer and tetramer with shift to the tetrameric state in presence of substrate | Aquifex aeolicus |
| 2.8.1.1 | tetramer | 4 * 12804, calculated, 4 * 12800, mass spectrometry, enzyme is in equilibrium between monomer, dimer and tetramer with shift to the tetrameric state in presence of substrate | Aquifex aeolicus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 2.8.1.1 | Aq-477 | - |
Aquifex aeolicus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.8.1.1 | 80 | - |
- |
Aquifex aeolicus |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.8.1.1 | 25 | - |
8% of maximum activity | Aquifex aeolicus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.8.1.1 | 9 | - |
- |
Aquifex aeolicus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.8.1.1 | 7 | - |
no residual activity below | Aquifex aeolicus |
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