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Literature summary extracted from

  • Li, J.; Huang, S.; Chen, J.; Yang, Z.; Fei, X.; Zheng, M.; Ji, C.; Xie, Y.; Mao, Y.
    Identification and characterization of human uracil phosphoribosyltransferase (UPRTase) (2007), J. Hum. Genet., 52, 415-422.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.9 cloning from a fetal brain cDNA library, DNA and amino acid sequence determination, subcloning in Escherichia coli strain DH5alpha, expression of His6-tagged enzyme in Escherichia coli strain M15, phylogenetic analysis, expressionin AD293 cells, tissue and subcellular expression pattern Homo sapiens
2.4.2.9 expressed in Escherichia coli strain M15 and in AD-293 cells Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.2.9 cytoplasm
-
 Homo sapiens 5737
-
2.4.2.9 nucleus
-
 Homo sapiens 5634
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.9 33800
-
 calculated from amino acid sequence Homo sapiens
2.4.2.9 38500
-
 x * 38500, recombinant His6-tagged enzyme, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.9 uracil + 5-phospho-alpha-D-ribose 1-diphosphate Homo sapiens
-
 UMP + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.9 Homo sapiens
-
-
-
2.4.2.9 Homo sapiens Q96BW1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.9 Ni-NTA affinity column chromatography Homo sapiens
2.4.2.9 recombinant His6-tagged enzyme from Escherichia coli strain M15 by nickel affinity chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.2.9 brain
-
 Homo sapiens
-
2.4.2.9 brain low level of expression Homo sapiens
-
2.4.2.9 heart
-
 Homo sapiens
-
2.4.2.9 heart low level of expression Homo sapiens
-
2.4.2.9 leukocyte high level of expression Homo sapiens
-
2.4.2.9 leukocyte high expression level Homo sapiens
-
2.4.2.9 liver high level of expression Homo sapiens
-
2.4.2.9 liver high expression level Homo sapiens
-
2.4.2.9 lung
-
 Homo sapiens
-
2.4.2.9 lung low level of expression Homo sapiens
-
2.4.2.9 additional information quantitative analysis of the tissue expression pattern Homo sapiens
-
2.4.2.9 prostate gland
-
 Homo sapiens
-
2.4.2.9 prostate gland low level of expression Homo sapiens
-
2.4.2.9 skeletal muscle
-
 Homo sapiens
-
2.4.2.9 skeletal muscle low level of expression Homo sapiens
-
2.4.2.9 spleen high level of expression Homo sapiens
-
2.4.2.9 spleen high expression level Homo sapiens
-
2.4.2.9 thymus high level of expression Homo sapiens
-
2.4.2.9 thymus high expression level Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.9 uracil + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Homo sapiens UMP + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.9 ? x * 38500, recombinant His6-tagged enzyme, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.4.2.9 UPRTase
-
 Homo sapiens

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.4.2.9 Homo sapiens calculated from amino acid sequence
-
 5.85
2.4.2.9 Homo sapiens sequence calculation
-
 5.85