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Literature summary extracted from

  • Chu, P.H.; Huang, T.Y.; Williams, J.; Stafford, D.W.
    Purified vitamin K epoxide reductase alone is sufficient for conversion of vitamin K epoxide to vitamin K and vitamin K to vitamin KH2 (2006), Proc. Natl. Acad. Sci. USA, 103, 19308-19313.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.17.4.4 overexpression of VKOR in Spodoptera frugiperda Sf9 cells using the baculovirus transfection system Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.17.4.4 warfarin
-
 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.17.4.4 membrane integral protein Homo sapiens 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.17.4.4 21000
-
 x * 21000, recombinant enzyme, SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.17.4.4 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.4.4 recombinant enzyme from Sf9 insect cell microsomes by ultracentrifugation, followed by HPC4 affinity chromatography in presence of DHPC and reconstitution in mixed detergent micelles, method optimization Homo sapiens

Renatured (Commentary)

EC Number Renatured (Comment) Organism
1.17.4.4 reconstitution of purified recombinant enzyme using 0.4% DOPC/0.4% deoxycholate/2 mM CaCl2/4 mM THP in 25 mM 3-(Nmorpholino)-propanesulfonic acid, 150 mM NaCl, and 20%glycerol at pH 7.5, method optimization Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.4.4 2,3-epoxy-2,3-dihydro-2-methyl-3-phytyl-1,4-naphthoquinone + 1,4-dithiothreitol i.e. vitamin K 2,3-epoxide Homo sapiens 2-hydroxy-2-methyl-3-phytyl-2,3-dihydronaphthoquinone + oxidized dithiothreitol i.e. vitamin K ?
1.17.4.4 2-methyl-3-phytyl-1,4-naphthoquinone + dithiothreitol i.e. vitamin K Homo sapiens vitamin K hydroquinone + oxidized dithiothreitol
-
 ?
1.17.4.4 additional information Purified vitamin K epoxide reductase alone is sufficient for conversion of vitamin K epoxide to vitamin K and vitamin K to vitamin KH2 Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.17.4.4 ? x * 21000, recombinant enzyme, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
1.17.4.4 vitamin K epoxid reductase
-
 Homo sapiens
1.17.4.4 Vitamin K reductase
-
 Homo sapiens
1.17.4.4 VKOR
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.17.4.4 30
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.17.4.4 8.6
-
 assay at Homo sapiens