EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.4.2.64 | xanthine | for the mutant enzyms D143S and D143T, xanthine is competitive, with respect to guanine | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.2.64 | 0.0026 | - |
xanthine | 37°C, pH 7.3, mutant enzyme D143N | Escherichia coli | |
2.4.2.64 | 0.0114 | - |
guanine | 37°C, pH 7.3, mutant enzyme D143A | Escherichia coli | |
2.4.2.64 | 0.013 | - |
xanthine | 37°C, pH 7.3, mutant enzyme D143A | Escherichia coli | |
2.4.2.64 | 0.0218 | - |
guanine | 37°C, pH 7.3, mutant enzyme D143N | Escherichia coli | |
2.4.2.64 | 0.057 | - |
guanine | 37°C, pH 7.3, mutant enzyme D143S | Escherichia coli | |
2.4.2.64 | 0.105 | - |
guanine | 37°C, pH 7.3, mutant enzyme D143T | Escherichia coli | |
2.4.2.64 | 0.224 | - |
xanthine | 37°C, pH 7.3, wild-type enzyme | Escherichia coli | |
2.4.2.64 | 0.49 | - |
hypoxanthine | 37°C, pH 7.3, mutant enzyme D143N | Escherichia coli | |
2.4.2.64 | 0.8 | 11 | hypoxanthine | 37°C, pH 7.3, wild-type enzyme | Escherichia coli | |
2.4.2.64 | 1.55 | - |
hypoxanthine | 37°C, pH 7.3, mutant enzyme D143A | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.2.64 | Escherichia coli | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.2.64 | tRNAqueuine + guanine | - |
Escherichia coli | tRNAguanine + queuine | - |
? | |
2.4.2.64 | tRNAqueuine + hypoxanthine | - |
Escherichia coli | tRNAhypoxanthine + queuine | - |
? | |
2.4.2.64 | tRNAqueuine + xanthine | - |
Escherichia coli | tRNAxanthine + queuine | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.2.64 | TGT | - |
Escherichia coli |
2.4.2.64 | tRNA-guanine transglycosylase | - |
Escherichia coli |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.2.64 | 0.0000055 | - |
xanthine | 37°C, pH 7.3, mutant enzyme D143A | Escherichia coli | |
2.4.2.64 | 0.0000088 | - |
xanthine | 37°C, pH 7.3, mutant enzyme D143N | Escherichia coli | |
2.4.2.64 | 0.000069 | - |
xanthine | 37°C, pH 7.3, wild-type enzyme | Escherichia coli | |
2.4.2.64 | 0.00012 | - |
hypoxanthine | 37°C, pH 7.3, mutant enzyme D143N | Escherichia coli | |
2.4.2.64 | 0.00019 | - |
hypoxanthine | 37°C, pH 7.3, mutant enzyme D143A | Escherichia coli | |
2.4.2.64 | 0.00297 | - |
hypoxanthine | 37°C, pH 7.3, wild-type enzyme | Escherichia coli |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.2.64 | 0.09 | - |
xanthine | 37°C, pH 7.3, mutant enzyme D143T | Escherichia coli | |
2.4.2.64 | 0.12 | - |
xanthine | 37°C, pH 7.3, mutant enzyme D143S | Escherichia coli |