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Literature summary extracted from

  • Consalvi, V.; Chiaraluce, R.; Politi, L.; Pasquo, A.; De Rosa, M.; Scandurra, R.
    Glutamate dehydrogenase from the thermoacidophilic archaebacterium Sulfolobus solfataricus: studies on thermal and guanidine-dependent inactivation. (1993), Biochim. Biophys. Acta, 1202, 207-215.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.4.1.3 guanidine-dependent inactivation of the enzyme at 20°C is irreversible above 1.5 M guanidine hydrochloride Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.3 45000
-
 x * 45000, SDS-PAGE Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.3 Saccharolobus solfataricus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.3 2-oxoglutarate + NADPH + NH3
-
 Saccharolobus solfataricus L-glutamate + NADP+ + H2O
-
 r

Subunits

EC Number Subunits Comment Organism
1.4.1.3 ? x * 45000, SDS-PAGE Saccharolobus solfataricus

Synonyms

EC Number Synonyms Comment Organism
1.4.1.3 NAD(P)-dependent glutamate dehydrogenase
-
 Saccharolobus solfataricus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.1.3 90
-
 incubation for 1 h at 0.2 mg/ml results in 90% loss of activity, incubation for 24 h at 0.8 mg/ml results in 30% loss of activity. Inactivation is irreversible. 3 M guanidine-HCl increases the half-life of the enzyme at 90°C and 0.2 mg/ml 6fold. Half-life at 90°C and 0.2 mg/ml protein concentration increases more than 6fold in the presence of 0.4 M Na2SO4 and decreases 4fold in the presence of 0.4 M NaSCN Saccharolobus solfataricus
1.4.1.3 95
-
 half-life: 15 min at 0.4 mg/ml, 30 min at 0.8 mg/ml. Temperature-dependent inactivation of the enzyme is irreversible, this process is accompanied by a progressive increase in hydrophobic surface area which leads to protein precipitation Saccharolobus solfataricus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.4.1.3 5.2 6.8 maximal stablility Saccharolobus solfataricus