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Literature summary extracted from

  • Park, Y.; Choi, S.Y.; Lee, H.
    A carboxylesterase from the thermoacidophilic archaeon Sulfolobus solfataricus P1; purification, characterization, and expression (2006), Biochim. Biophys. Acta, 1760, 820-828.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.1 expression in Escherichia coli Saccharolobus solfataricus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.1 additional information enzyme retains 97% of activity after 60 min in 1 M urea. Not inhibitory: 1% Tween 20, 1% Lubrol Saccharolobus solfataricus
3.1.1.1 sodium dodecylsulfate 1%, 60 min at 30°C, 59% residual activity Saccharolobus solfataricus
3.1.1.1 Triton X-100 1%, 60 min at 30°C, 81% residual activity Saccharolobus solfataricus
3.1.1.1 Tween 80 1%, 60 min at 30°C, 76% residual activity Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.1 34000
-
 gel filtration Saccharolobus solfataricus

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.1.1.1 2-propanol 40%, 60 min at 30°C, 91% residual activity Saccharolobus solfataricus
3.1.1.1 Acetone 40%, 60 min at 30°C, 61% residual activity Saccharolobus solfataricus
3.1.1.1 Ethanol 40%, 60 min at 30°C, 90% residual activity Saccharolobus solfataricus
3.1.1.1 Methanol 40%, 60 min at 30°C, 86% residual activity Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.1 Saccharolobus solfataricus
-
-
-
3.1.1.1 Saccharolobus solfataricus P1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.1
-
 Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.1 3975
-
 pH 7.0, 50°C Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.1 4-nitrophenyl palmitate + H2O
-
 Saccharolobus solfataricus 4-nitrophenol + palmitate
-
 ?
3.1.1.1 4-nitrophenyl palmitate + H2O
-
 Saccharolobus solfataricus P1 4-nitrophenol + palmitate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.1 monomer 1 * 34000, SDS-PAGE, MALDI-TOF Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.1 85
-
-
 Saccharolobus solfataricus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.1 80
-
 5 days, 41% residual activity Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.1 8
-
-
 Saccharolobus solfataricus