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Literature summary extracted from

  • Guimaraes, L.H.; Junior, A.B.; Jorge, J.A.; Terenzi, H.F.; Polizeli, M.L.
    Purification and biochemical characterization of a mycelial alkaline phosphatase without DNAase activity produced by Aspergillus caespitosus (2007), Folia Microbiol. (Praha), 52, 231-236.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.1 NH4Cl 10 mM, activity is enhanced to 165% of control Aspergillus caespitosus

Application

EC Number Application Comment Organism
3.1.3.1 molecular biology the purified alkaline phosphatase removes the 5'-phosphate group of a linearized plasmid without showing DNAase activity, indicating its potential for recombinant DNA technology Aspergillus caespitosus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.1 CaCl2 1 mM, 32% inhibition Aspergillus caespitosus
3.1.3.1 CuCl2 10 mM, 24% inhibition Aspergillus caespitosus
3.1.3.1 CuSO4 10 mM, 71% inhibition Aspergillus caespitosus
3.1.3.1 EDTA 10 mM, 71% inhibition Aspergillus caespitosus
3.1.3.1 HgCl2 10 mM, 74%inhibition Aspergillus caespitosus
3.1.3.1 ZnCl2 10 mM, 58% inhibition Aspergillus caespitosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.1 0.27
-
 4-nitrophenyl phosphate without Mg2+ Aspergillus caespitosus
3.1.3.1 0.41
-
 4-nitrophenyl phosphate with Mg2+ Aspergillus caespitosus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.1 Mg2+ 10 mM MgSO4, activity is enhanced to 239% of control Aspergillus caespitosus
3.1.3.1 MnCl2 10 mM, activity is enhanced to 138% of control Aspergillus caespitosus
3.1.3.1 NaCl 10 mM, activity is enhanced to 151% of control Aspergillus caespitosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.1 71000
-
 2 * 71000, SDS-PAGE Aspergillus caespitosus
3.1.3.1 138000
-
 gel filtration Aspergillus caespitosus

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.1 Aspergillus caespitosus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.3.1 glycoprotein 74% saccharide content Aspergillus caespitosus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.1
-
 Aspergillus caespitosus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.1 mycelium
-
 Aspergillus caespitosus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.1 50.4
-
-
 Aspergillus caespitosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.1 2-phosphoglycerate + H2O 53.7% of the activity with 4-nitrophenyl phosphate Aspergillus caespitosus glycerol + phosphate
-
 ?
3.1.3.1 3'-ITP + H2O 51.5% of the activity with 4-nitrophenyl phosphate Aspergillus caespitosus ?
-
 ?
3.1.3.1 4-nitrophenyl phosphate + H2O
-
 Aspergillus caespitosus 4-nitrophenol + phosphate
-
 ?
3.1.3.1 adenosine 5'-(beta-glucosyl)diphosphate + H2O 29.8% of the activity with 4-nitrophenyl phosphate Aspergillus caespitosus ?
-
 ?
3.1.3.1 ATP + H2O 57.8% of the activity with 4-nitrophenyl phosphate Aspergillus caespitosus ?
-
 ?
3.1.3.1 D-fructose 6-phosphate + H2O 5.2% of the activity with 4-nitrophenyl phosphate Aspergillus caespitosus D-fructose + phosphate
-
 ?
3.1.3.1 D-glucose 6-phosphate + H2O 19.4% of the activity with 4-nitrophenyl phosphate Aspergillus caespitosus D-glucose + phosphate
-
 ?
3.1.3.1 glucose 1-phosphate + H2O 93% of the activity with 4-nitrophenyl phosphate Aspergillus caespitosus glucose + phosphate
-
 ?
3.1.3.1 additional information the purified alkaline phosphatase removes the 5'-phosphate group of a linearized plasmid without showing DNAase activity Aspergillus caespitosus ?
-
 ?
3.1.3.1 phosphoserine + H2O 9.3% of the activity with 4-nitrophenyl phosphate Aspergillus caespitosus phosphate + Ser
-
 ?
3.1.3.1 phosphothreonine + H2O 22.1% of the activity with 4-nitrophenyl phosphate Aspergillus caespitosus phosphate + Thr
-
 ?
3.1.3.1 phosphotyrosine + H2O 23.7% of the activity with 4-nitrophenyl phosphate Aspergillus caespitosus phosphate + Tyr
-
 ?
3.1.3.1 UTP + H2O 67% of the activity with 4-nitrophenyl phosphate Aspergillus caespitosus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.1 dimer 2 * 71000, SDS-PAGE Aspergillus caespitosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.1 80
-
-
 Aspergillus caespitosus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.3.1 65 85 65°C: about 55% of maximal activity, 85°C: about 55% maximal activity Aspergillus caespitosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.1 60
-
 60 min, about 5% of maximal activity Aspergillus caespitosus
3.1.3.1 70
-
 60 min, about 50% loss of activity Aspergillus caespitosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.1 9
-
-
 Aspergillus caespitosus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.1 8 10.5 pH 8.0: about 65% of maximal activity, pH 10.5: about 95% of maximal activity Aspergillus caespitosus