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Literature summary extracted from

  • Morana, A.; Di Prizito, N.; Aurilia, V.; Rossi, M.; Cannio, R.
    A carboxylesterase from the hyperthermophilic archaeon Sulfolobus solfataricus: cloning of the gene, characterization of the protein. (2002), Gene, 283, 107-115.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.1 expression in Escherichia coli Saccharolobus solfataricus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.1 dithiothreitol 1 mM, 35% inhibition Saccharolobus solfataricus
3.1.1.1 phenylmethylsulfonyl fluoride 1 mM, complete inhibition Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.1 32000
-
 4 * 32000, SDS-PAGE Saccharolobus solfataricus
3.1.1.1 33425
-
 4 * 33425, calculated from sequence Saccharolobus solfataricus
3.1.1.1 114000
-
 gel filtration Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.1 Saccharolobus solfataricus
-
-
-
3.1.1.1 Saccharolobus solfataricus MT4
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.1 partial Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.1 2-naphthyl acetate + H2O
-
 Saccharolobus solfataricus 2-naphthol + acetate
-
 ?
3.1.1.1 2-naphthyl acetate + H2O
-
 Saccharolobus solfataricus MT4 2-naphthol + acetate
-
 ?
3.1.1.1 4-nitrophenyl acetate + H2O at 37°C, 14% of the activity with pentanoyl-CoA Saccharolobus solfataricus 4-nitrophenol + acetate
-
 ?
3.1.1.1 4-nitrophenyl acetate + H2O at 37°C, 14% of the activity with pentanoyl-CoA Saccharolobus solfataricus MT4 4-nitrophenol + acetate
-
 ?
3.1.1.1 4-nitrophenyl butyrate + H2O at 37°C, 74% of the activity with pentanoyl-CoA Saccharolobus solfataricus 4-nitrophenol + butyrate
-
 ?
3.1.1.1 4-nitrophenyl butyrate + H2O at 37°C, 74% of the activity with pentanoyl-CoA Saccharolobus solfataricus MT4 4-nitrophenol + butyrate
-
 ?
3.1.1.1 4-nitrophenyl hexanoate + H2O at 37°C, 69% of the activity with pentanoyl-CoA Saccharolobus solfataricus 4-nitrophenol + hexanoate
-
 ?
3.1.1.1 4-nitrophenyl pentanoate + H2O
-
 Saccharolobus solfataricus 4-nitrophenol + pentanoate
-
 ?
3.1.1.1 4-nitrophenyl pentanoate + H2O
-
 Saccharolobus solfataricus MT4 4-nitrophenol + pentanoate
-
 ?
3.1.1.1 additional information no activity with triolein Saccharolobus solfataricus ?
-
 ?
3.1.1.1 additional information no activity with triolein Saccharolobus solfataricus MT4 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.1 tetramer 4 * 32000, SDS-PAGE Saccharolobus solfataricus
3.1.1.1 tetramer 4 * 33425, calculated from sequence Saccharolobus solfataricus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.1 70
-
 24 h, stable Saccharolobus solfataricus
3.1.1.1 80
-
 24 h, 50% loss of activity Saccharolobus solfataricus
3.1.1.1 90
-
 24 h, 85% loss of activity Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.1 6.5 7
-
 Saccharolobus solfataricus