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Literature summary extracted from

  • Bartolucci, S.; Rella, R.; Guagliardi, A.; Raia, C.A.; Gambacorta, A.; De Rosa, M.; Rossi, M.
    Malic enzyme from archaebacterium Sulfolobus solfataricus. Purification, structure, and kinetic properties. (1987), J. Biol. Chem., 262, 7725-7731.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.40 0.003
-
 NADP+ 60°C, pH 8.0 Saccharolobus solfataricus
1.1.1.40 0.018
-
 (S)-malate 60°C, pH 8.0, presence of Mn2+ Saccharolobus solfataricus
1.1.1.40 0.065
-
 (S)-malate 60°C, pH 8.0, presence of Mg2+ Saccharolobus solfataricus
1.1.1.40 0.25
-
 NAD+ 60°C, pH 8.0 Saccharolobus solfataricus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.40 Mg2+ may partly replace for Mn2+ Saccharolobus solfataricus
1.1.1.40 Mn2+ most effective divalent cation Saccharolobus solfataricus
1.1.1.40 additional information absolute requirement for a divalent cation. Ca2+ is barely effective Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.40 49000
-
 2 * 49000, SDS-PAGE Saccharolobus solfataricus
1.1.1.40 105000
-
 gel filtration Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.40 Saccharolobus solfataricus
-
 enzyme additionally catalyzes decarboxylation of oxalacetate
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.40
-
 Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.40 36.6
-
 60°C, pH 8.0 Saccharolobus solfataricus

Storage Stability

EC Number Storage Stability Organism
1.1.1.40 25°C, pH 8.0, stable for at least one month Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.40 (S)-malate + NAD+ NADP+ is preferred over NAD+ Saccharolobus solfataricus pyruvate + CO2 + NADH
-
 ?
1.1.1.40 (S)-malate + NADP+ NADP+ is preferred over NAD+ Saccharolobus solfataricus pyruvate + CO2 + NADPH
-
 ?
1.1.1.40 additional information enzyme additionally catalyzes decarboxylation of oxalacetate Saccharolobus solfataricus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.40 dimer 2 * 49000, SDS-PAGE Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.40 85
-
-
 Saccharolobus solfataricus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.40 60
-
 stable for at least 24 h Saccharolobus solfataricus
1.1.1.40 75
-
 stable for at least 12 h Saccharolobus solfataricus
1.1.1.40 80
-
 stable for at least 7 h Saccharolobus solfataricus
1.1.1.40 85
-
 stable for at least 5 h Saccharolobus solfataricus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.40 376
-
 (S)-malate pH 8.0, 60°C Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.40 8
-
-
 Saccharolobus solfataricus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.40 4.5
-
 stable for 24 h Saccharolobus solfataricus
1.1.1.40 8
-
 stable for at least one month Saccharolobus solfataricus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.40 NAD+
-
 Saccharolobus solfataricus
1.1.1.40 NADP+
-
 Saccharolobus solfataricus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.1.40 Saccharolobus solfataricus isoelectric focusing
-
 5.1