EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
4.6.1.2 | 5-cyclopropyl-2-[1-(2-fluorobenzyl)-1H-pyrazolo[3,4-b]pyridin-3-yl]-pyrimidin-4-ylamine | - |
Caldanaerobacter subterraneus subsp. tengcongensis | |
4.6.1.2 | CO | limited affinity | Caldanaerobacter subterraneus subsp. tengcongensis | |
4.6.1.2 | additional information | no affinity for O2, the mechanism of oxygen exclusion by sGC not only involves the lack of hydrogen bonding in the distal heme pocket, but also depends on structural elements from other domains of sGC | Caldanaerobacter subterraneus subsp. tengcongensis | |
4.6.1.2 | NO | - |
Caldanaerobacter subterraneus subsp. tengcongensis | |
4.6.1.2 | protoporphyrin IX | - |
Caldanaerobacter subterraneus subsp. tengcongensis |
EC Number | Cloned (Comment) | Organism |
---|---|---|
4.6.1.2 | expression of wild-type and mutant in Sf9 cells | Caldanaerobacter subterraneus subsp. tengcongensis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.6.1.2 | I145Y | substitution in the full-length beta-subunit of the sGC heterodimer, does not produce an oxygen-binding enzyme, but impedes the association of NO and destabilizes the NO-heme complex. The tyrosine in the distal heme pocket impedes both the binding and dissociation of the CO ligand. Mutation does not affect stimulatory effect of manganese ion on the activity of the enzyme, thus function of the catalytic domain is not directly affected | Caldanaerobacter subterraneus subsp. tengcongensis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.6.1.2 | Mn2+ | - |
Caldanaerobacter subterraneus subsp. tengcongensis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.6.1.2 | Caldanaerobacter subterraneus subsp. tengcongensis | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.6.1.2 | by gel filtration and on nickel-agarose column, at least 95% pure | Caldanaerobacter subterraneus subsp. tengcongensis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.6.1.2 | GTP | - |
Caldanaerobacter subterraneus subsp. tengcongensis | 3',5'-cyclic GMP + diphosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.6.1.2 | sGC | - |
Caldanaerobacter subterraneus subsp. tengcongensis |
4.6.1.2 | soluble guanylyl cyclase | - |
Caldanaerobacter subterraneus subsp. tengcongensis |