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Literature summary extracted from

  • Gould, T.A.; van de Langemheen, H.; Munoz-Elias, E.J.; McKinney, J.D.; Sacchettini, J.C.
    Dual role of isocitrate lyase 1 in the glyoxylate and methylcitrate cycles in Mycobacterium tuberculosis (2006), Mol. Microbiol., 61, 940-947.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.3.1 expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.3.1 vapour diffusion with 0.1 M Tris-HCl, 0.2 M sodium acetate, 20-30% (w/v) PEG 4000, pH 8.0 Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
4.1.3.1 C191A unable to form the closed conformation crystal form Mycobacterium tuberculosis
4.1.3.1 C195A reduced activity Mycobacterium tuberculosis
4.1.3.1 C195S reduced activity Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.3.1 0.188
-
 isocitrate ICL1, in 50 mM MOPS pH 6.8, 5 mM MgCl2 Mycobacterium tuberculosis
4.1.3.1 0.718
-
 (2R,3S:2S,3R)-2-methyl-isocitrate ICL1, in 50 mM MOPS pH 6.8, 5 mM MgCl2 Mycobacterium tuberculosis
4.1.3.1 1.14
-
 isocitrate ICL2, in Tricine-HCl pH 7.5, 5 mM MgCl2 Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.3.1 Mg2+ required Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.1 Mycobacterium tuberculosis P9WKK7
-
-
4.1.3.1 Mycobacterium tuberculosis H37Rv P9WKK7
-
-
4.1.3.30 Mycobacterium tuberculosis
-
-
-
4.2.1.79 Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.3.1 HiTrap Q FF ion exchange column chromatography, Superdex S-200 gel filtration, and HiTrap Q HP ion exchange column chromatography Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.1 (2R,3S:2S,3R)-2-methyl-isocitrate
-
 Mycobacterium tuberculosis succinate + pyruvate
-
 ?
4.1.3.1 (2R,3S:2S,3R)-2-methyl-isocitrate
-
 Mycobacterium tuberculosis H37Rv succinate + pyruvate
-
 ?
4.1.3.1 isocitrate
-
 Mycobacterium tuberculosis succinate + glyoxylate
-
 ?
4.1.3.1 isocitrate
-
 Mycobacterium tuberculosis H37Rv succinate + glyoxylate
-
 ?
4.1.3.30 (2S,3R)-3-Hydroxybutane-1,2,3-tricarboxylate
-
 Mycobacterium tuberculosis Pyruvate + succinate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.3.1 homotetramer x-ray crystallography Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
4.1.3.1 ICL1 isoenzyme, has both isocitrate lyase and 2-methylisocitrate lyase activity Mycobacterium tuberculosis
4.1.3.1 ICL2 isoenzyme, has no 2-methylisocitrate lyase activity Mycobacterium tuberculosis
4.1.3.30 2-Methylisocitrate lyase
-
 Mycobacterium tuberculosis
4.1.3.30 MCL
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.3.1 1.25
-
 (2R,3S:2S,3R)-2-methyl-isocitrate ICL1, in 50 mM MOPS pH 6.8, 5 mM MgCl2 Mycobacterium tuberculosis
4.1.3.1 1.38
-
 isocitrate ICL2, in Tricine-HCl pH 7.5, 5 mM MgCl2 Mycobacterium tuberculosis
4.1.3.1 5.24
-
 isocitrate ICL1, in 50 mM MOPS pH 6.8, 5 mM MgCl2 Mycobacterium tuberculosis