Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Lu, J.; Esberg, A.; Huang, B.; Bystroem, A.S.
    Kluyveromyces lactis gamma-toxin, a ribonuclease that recognizes the anticodon stem loop of tRNA (2008), Nucleic Acids Res., 36, 1072-1080.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.14.1 additional information strong stimulatory effects of the mcm5 group, weak positive effect of the s2 group and a negative effect of the bacterial 5-methylaminomethyl group Kluyveromyces lactis
3.1.14.1 Trimethylamine N-oxide enhances cleavage of unmodified tRNA and anticodon stem loops by gamma-toxin-GST Kluyveromyces lactis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.14.1 gamma-toxin-GST expressed in Escherichia coli BL21(DE3) Kluyveromyces lactis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.6.1.16 tRNA + H2O Escherichia coli
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.14.1 Kluyveromyces lactis
-
-
-
4.6.1.16 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.14.1
-
 Kluyveromyces lactis

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Storage Stability

EC Number Storage Stability Organism
3.1.14.1 -20°C, 50% glycerol, stable for 12 months Kluyveromyces lactis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.14.1 RNA + H2O in vitro transcribed tRNAGlu3 UUC, tRNAGIn UUG and tRNALys UUU are substrates, stable anticodon stem and the anticodon loop are the minimal requirements for cleavage, synthetic minihelix RNA corresponding to the anticodon stem loop of the natural substrate tRNAGlu3 mcm5s2UUC is cleaved at the same position as the natural substrate. In ASLGlu3 UUC, the nucleotides U34U35C36A37C38 are required for optimal gamma-toxin cleavage, whereas a purine at position 32 or a G in position 33 dramatically reduces the cleavage of the anticodon stem loop Kluyveromyces lactis nucleoside 3'-phosphate + ?
-
 ?
4.6.1.16 tRNA + H2O
-
 Escherichia coli ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.14.1 Gamma-toxin
-
 Kluyveromyces lactis
3.1.14.1 tRNA endonuclease
-
 Kluyveromyces lactis
4.6.1.16 Gamma-toxin
-
 Escherichia coli
4.6.1.16 tRNA anticodon nucleases (ACNs)
-
 Escherichia coli