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Literature summary extracted from

  • Ohshiro, T.; Ohkita, R.; Takikawa, T.; Manabe, M.; Lee, W.C.; Tanokura, M.; Izumi, Y.
    Improvement of 2-hydroxybiphenyl-2-sulfinate desulfinase, an enzyme involved in the dibenzothiophene desulfurization pathway, from Rhodococcus erythropolis KA2-5-1 by site-directed mutagenesis (2007), Biosci. Biotechnol. Biochem., 71, 2815-2821.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.13.1.3 expression in Escherichia coli Rhodococcus erythropolis

Protein Variants

EC Number Protein Variants Comment Organism
3.13.1.3 Q65H increase in thermostability, decrease in catalytic activity Rhodococcus erythropolis
3.13.1.3 Y63A increase in catalytic activity Rhodococcus erythropolis
3.13.1.3 Y63F increase in catalytic activity, similar thermostability as wild-type Rhodococcus erythropolis
3.13.1.3 Y63F/Q65H increase in thermostability without loss of catalytic activity or affinity for substrate Rhodococcus erythropolis
3.13.1.3 Y63K decrease in activity Rhodococcus erythropolis
3.13.1.3 Y63S affinity for the substrate is about 7-fold lower than that of the wild-type enzyme Rhodococcus erythropolis
3.13.1.3 Y63W activity similar to wild-type Rhodococcus erythropolis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.13.1.3 0.0056
-
 2'-hydroxybiphenyl-2-sulfinate mutant Y63F, pH 7.0, 28°C Rhodococcus erythropolis
3.13.1.3 0.0082
-
 2'-hydroxybiphenyl-2-sulfinate wild-type, pH 7.0, 28°C Rhodococcus erythropolis
3.13.1.3 0.0098
-
 2'-hydroxybiphenyl-2-sulfinate mutant Y63F/Q65H, pH 7.0, 28°C Rhodococcus erythropolis
3.13.1.3 0.0556
-
 2'-hydroxybiphenyl-2-sulfinate mutant Y63S, pH 7.0, 28°C Rhodococcus erythropolis

Organism

EC Number Organism UniProt Comment Textmining
3.13.1.3 Rhodococcus erythropolis P54997 strain KA2-5-1
-
3.13.1.3 Rhodococcus erythropolis IGTS8 / ATCC 53968 P54997 strain KA2-5-1
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.13.1.3 8.3
-
 mutant Y63K, pH 7.0, 28°C Rhodococcus erythropolis
3.13.1.3 21
-
 mutant Q65H, pH 7.0, 28°C Rhodococcus erythropolis
3.13.1.3 26.8
-
 wild-type, pH 7.0, 28°C Rhodococcus erythropolis
3.13.1.3 29.2
-
 mutant Y63W, pH 7.0, 28°C Rhodococcus erythropolis
3.13.1.3 34.1
-
 mutant Y63F/Q65H, pH 7.0, 28°C Rhodococcus erythropolis
3.13.1.3 43
-
 mutant Y63F, pH 7.0, 28°C Rhodococcus erythropolis
3.13.1.3 49.1
-
 mutant Y63A, pH 7.0, 28°C Rhodococcus erythropolis
3.13.1.3 61.1
-
 mutant Y63S, pH 7.0, 28°C Rhodococcus erythropolis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.13.1.3 2'-hydroxybiphenyl-2-sulfinate + H2O
-
 Rhodococcus erythropolis 2-hydroxybiphenyl + sulfite
-
 ?
3.13.1.3 2'-hydroxybiphenyl-2-sulfinate + H2O
-
 Rhodococcus erythropolis IGTS8 / ATCC 53968 2-hydroxybiphenyl + sulfite
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.13.1.3 35
-
 30 min, wild-type, almost complete loss of activity, mutant Y63F/Q65H, 90% residual activity Rhodococcus erythropolis
3.13.1.3 40
-
 30 min,mutant Y63F/Q65H, 90% residual activity Rhodococcus erythropolis