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Literature summary extracted from

  • Busenlehner, L.S.; Alander, J.; Jegerscoehld, C.; Holm, P.J.; Bhakat, P.; Hebert, H.; Morgenstern, R.; Armstrong, R.N.
    Location of substrate binding sites within the integral membrane protein microsomal glutathione transferase-1 (2007), Biochemistry, 46, 2812-2822.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.18 expressed in Escherichia coli BL21(DE3)pLysSL cells Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.18 E80Q 7% residual activity compared to the wild type enzyme Rattus norvegicus
2.5.1.18 H75Q 34% residual activity compared to the wild type enzyme Rattus norvegicus
2.5.1.18 N77T inactive Rattus norvegicus
2.5.1.18 R113K 5% residual activity compared to the wild type enzyme Rattus norvegicus
2.5.1.18 R63A 30% increased activity compared to the wild type enzyme Rattus norvegicus
2.5.1.18 R72A 42% residual activity compared to the wild type enzyme Rattus norvegicus
2.5.1.18 R73Q 57% residual activity compared to the wild type enzyme Rattus norvegicus
2.5.1.18 S30A 400% increased activity compared to the wild type enzyme Rattus norvegicus
2.5.1.18 T64V 280% increased activity compared to the wild type enzyme Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.18 membrane
-
 Rattus norvegicus 16020
-
2.5.1.18 microsome
-
 Rattus norvegicus
-
-

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.18 Rattus norvegicus P08011
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.18
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.18 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.18 5.6
-
 wild type enzyme, using 1-chloro-2,4-dinitrobenzene as a substrate Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.18 glutathione + 1-chloro-2,4-dinitrobenzene
-
 Rattus norvegicus S-(2,4-dinitrophenyl)glutathione + HCl
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.18 trimer
-
 Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
2.5.1.18 MGST1
-
 Rattus norvegicus
2.5.1.18 microsomal glutathione transferase-1 membrane-bound enzyme with both glutathione transferase and hydroperoxidase activities Rattus norvegicus