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Literature summary extracted from

  • Ikegami, T.; Kameyama, E.; Yamamoto, S.Y.; Minami, Y.; Yubisui, T.
    Structure and properties of the recombinant NADH-cytochrome b5 reductase of Physarum polycephalum (2007), Biosci. Biotechnol. Biochem., 71, 783-790.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.6.2.2
-
 Physarum polycephalum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.2.2 0.002
-
 NADH
-
 Physarum polycephalum
1.6.2.2 0.014
-
 ferricytochrome b5
-
 Physarum polycephalum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.6.2.2 29000
-
 x * 29000, SDS-PAGE Physarum polycephalum

Organism

EC Number Organism UniProt Comment Textmining
1.6.2.2 Physarum polycephalum Q1HA49
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.2.2 recombinant enzyme Physarum polycephalum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6.2.2 48.4
-
-
 Physarum polycephalum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.2.2 2 ferricytochrome b5 + NADH
-
 Physarum polycephalum 2 ferrocytochrome b5 + NAD+ + H+
-
 ?
1.6.2.2 2 ferricytochrome b5 + NADPH with NADPH the enzyme shows about 20% of the activity with NADH Physarum polycephalum 2 ferrocytochrome b5 + NADP+ + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.6.2.2 ? x * 29000, SDS-PAGE Physarum polycephalum

Synonyms

EC Number Synonyms Comment Organism
1.6.2.2 NADH-cytochrome b5 reductase
-
 Physarum polycephalum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.6.2.2 40
-
 30 min, less than 10% loss of activity Physarum polycephalum
1.6.2.2 50
-
 30 min, 30 min, about 80% loss of activity Physarum polycephalum
1.6.2.2 55
-
 10 min, complete loss of activity Physarum polycephalum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.6.2.2 6
-
 around, recombinant enzyme Physarum polycephalum

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.2.2 FAD
-
 Physarum polycephalum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.6.2.2 Physarum polycephalum calculated from sequence
-
 8.9