EC Number | Cloned (Comment) | Organism |
---|---|---|
3.5.4.43 | gene atzB, expression in Escherichia coli strain DH5alpha | Pseudomonas sp. |
3.8.1.8 | using the vectors pACYC184 and pMD4 for expression in Escherichia coli DH5alpha cells | Pseudomonas sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.5.4.43 | 1,10-phenanthroline | 72% inhibition, not due to metal removal | Pseudomonas sp. | |
3.5.4.43 | 8-Hydroxyquinoline-5-sulfonic acid | - |
Pseudomonas sp. | |
3.5.4.43 | Chelex-100 resin | - |
Pseudomonas sp. | |
3.5.4.43 | dipicolinic acid | - |
Pseudomonas sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.8.1.8 | 0.02 | - |
hydroxyatrazine | - |
Pseudomonas sp. | |
3.8.1.8 | 0.02 | - |
2-(N-ethylamino)-4-hydroxy-6-(N-isopropylamino)-1,3,5-triazine | deamination | Pseudomonas sp. | |
3.8.1.8 | 0.029 | - |
2,4-diisopropylamino-6-hydroxy-1,3,5-triazine | deamination | Pseudomonas sp. | |
3.8.1.8 | 0.04 | - |
6-hydroxy-4-(N-isopropylamino)-2-(N-methylamino)-1,3,5-triazine | deamination | Pseudomonas sp. | |
3.8.1.8 | 0.12 | - |
2-chloro-4-hydroxy-6-(N-isopropylamino)-1,3,5-triazine | dechlorination | Pseudomonas sp. | |
3.8.1.8 | 0.23 | - |
2-chloro-4-(N-ethylamino)-6-hydroxy-1,3,5-triazine | dechlorination | Pseudomonas sp. |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.8.1.8 | Zn2+ | - |
Pseudomonas sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.5.4.43 | 52000 | - |
2 * 52000, recombinant AtzB, SDS-PAGE | Pseudomonas sp. |
3.5.4.43 | 105000 | - |
recombinant AtzB, gel filtration | Pseudomonas sp. |
3.8.1.8 | 52000 | - |
subunit molecular mass, determind by SDS-Page | Pseudomonas sp. |
3.8.1.8 | 105000 | - |
holoenzyme molecular mass, determind by gel-filtration | Pseudomonas sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.8.1.8 | hydroxyatrazine + H2O | Pseudomonas sp. | - |
N-isopropylammelide + ethylamine | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.4.43 | Pseudomonas sp. | - |
gene atzB | - |
3.8.1.8 | Pseudomonas sp. | - |
Pseudomonas sp. starin ADP | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.5.4.43 | recombinant enzyme 6.6fold from Escherichia coli strain DH5alpha to homogeneity by ammonium sulfate fractionation, hydroxyapatite chromatography, and gel filtration | Pseudomonas sp. |
3.8.1.8 | using a CHT ceramic hydroxyapatite type I column | Pseudomonas sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.5.4.43 | 1.05 | - |
purified recombinant enzyme | Pseudomonas sp. |
3.8.1.8 | 0.16 | - |
crude extract | Pseudomonas sp. |
3.8.1.8 | 0.33 | - |
prurification step ammonium sulfate precipitation | Pseudomonas sp. |
3.8.1.8 | 1.05 | - |
prurification step ceramic hydroxyapatite type I column | Pseudomonas sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.4.43 | 2,4-di(N-isobutylamino)-6-hydroxy-1,3,5-triazine + H2O | - |
Pseudomonas sp. | ? | - |
? | |
3.5.4.43 | 2,4-di(N-sec-butylamino)-6-hydroxy-1,3,5-triazine + H2O | - |
Pseudomonas sp. | ? | - |
? | |
3.5.4.43 | 2,4-di(N-tert-butylamino)-6-hydroxy-1,3,5-triazine + H2O | - |
Pseudomonas sp. | ? | - |
? | |
3.5.4.43 | 2,4-diamino-6-hydroxy-1,3,5-triazine + H2O | - |
Pseudomonas sp. | ? | - |
? | |
3.5.4.43 | 2,4-dichloro-6-amino-1,3,5-triazine + H2O | - |
Pseudomonas sp. | ? | - |
? | |
3.5.4.43 | 2,4-diethylamino-6-hydroxy-1,3,5-triazine + H2O | - |
Pseudomonas sp. | ? | - |
? | |
3.5.4.43 | 2,4-diisopropylamino-6-hydroxy-1,3,5-triazine + H2O | - |
Pseudomonas sp. | ? | - |
? | |
3.5.4.43 | 2,4-dimethylamino-6-hydroxy-1,3,5-triazine + H2O | - |
Pseudomonas sp. | ? | - |
? | |
3.5.4.43 | 2-(N-ethyl-N-methylamino)-4-ethylamino-6-hydroxy-1,3,5-triazine + H2O | - |
Pseudomonas sp. | ? | - |
? | |
3.5.4.43 | 2-(N-ethylamino)-4-hydroxy-6-(N-hydroxyethylamino)-1,3,5-triazine + H2O | - |
Pseudomonas sp. | ? | - |
? | |
3.5.4.43 | 2-(N-ethylamino)-4-hydroxy-6-(N-isopropylamino)-1,3,5-triazine + H2O | - |
Pseudomonas sp. | ? | - |
? | |
3.5.4.43 | 2-amino-4-(N-ethylamino)-6-(N-isopropylamino)-1,3,5-triazine + H2O | - |
Pseudomonas sp. | ? | - |
? | |
3.5.4.43 | 2-amino-4-chloro-6-hydroxy-1,3,5-triazine + H2O | - |
Pseudomonas sp. | ? | - |
? | |
3.5.4.43 | 2-amino-4-hydroxy-6-(N-hydroxyethylamino)-1,3,5-triazine + H2O | - |
Pseudomonas sp. | ? | - |
? | |
3.5.4.43 | 2-amino-4-hydroxy-6-(N-isopropylamino)-1,3,5-triazine + H2O | - |
Pseudomonas sp. | ? | - |
? | |
3.5.4.43 | 2-amino-6-(N-ethylamino)-4-hydroxy-1,3,5-triazine + H2O | - |
Pseudomonas sp. | ? | - |
? | |
3.5.4.43 | 2-azido-4-(N-ethylamino)-6-(N-isopropylamino)-1,3,5-triazine + H2O | - |
Pseudomonas sp. | ? | - |
? | |
3.5.4.43 | 2-chloro-4-(N-ethylamino)-6-hydroxy-1,3,5-triazine + H2O | - |
Pseudomonas sp. | ? | - |
? | |
3.5.4.43 | 2-chloro-4-amino-6-hydroxy-1,3,5-triazine + H2O | - |
Pseudomonas sp. | ? | - |
? | |
3.5.4.43 | 2-chloro-4-hydroxy-6-(N-isopropylamino)-1,3,5-triazine + H2O | - |
Pseudomonas sp. | ? | - |
? | |
3.5.4.43 | 2-cyano-4-(N-ethylamino)-6-(N-isopropylamino)-1,3,5-triazine + H2O | - |
Pseudomonas sp. | ? | - |
? | |
3.5.4.43 | 2-fluoro-4-(N-ethylamino)-6-(N-isopropylamino)-1,3,5-triazine + H2O | - |
Pseudomonas sp. | ? | - |
? | |
3.5.4.43 | 2-hydroxy-4,6-di(N-hydroxyethylamino)-1,3,5-triazine + H2O | - |
Pseudomonas sp. | ? | - |
? | |
3.5.4.43 | 2-hydroxy-4-(N-ethylamino)-6-(N-isopropylamino)-1,3,5-triazine + H2O | - |
Pseudomonas sp. | ? | - |
? | |
3.5.4.43 | 2-hydroxy-4-(N-isopropylamino)-6-(N-(3-methoxypropyl)amino)-1,3,5-triazine + H2O | - |
Pseudomonas sp. | ? | - |
? | |
3.5.4.43 | 2-isopropylamino-4-ethylamino-1,3,5-triazine + H2O | - |
Pseudomonas sp. | ? | - |
? | |
3.5.4.43 | 2-mercapto-4-isopropylamino-6-ethylamino-1,3,5-triazine + H2O | - |
Pseudomonas sp. | ? | - |
? | |
3.5.4.43 | 4-(N-ethylamino)-6-hydroxy-2-(N-methylamino)-1,3,5-triazine + H2O | - |
Pseudomonas sp. | ? | - |
? | |
3.5.4.43 | 6-hydroxy-4-(N-isopropylamino)-2-(N-methylamino)-1,3,5-triazine + H2O | - |
Pseudomonas sp. | ? | - |
? | |
3.5.4.43 | additional information | the enzyme performs deamination and dechlorination, substrate specificity, overview | Pseudomonas sp. | ? | - |
? | |
3.8.1.8 | 2,4-di(N-isobutylamino)-6-hydroxy-1,3,5-triazine + H2O | - |
Pseudomonas sp. | 6-[(2-methylpropyl)amino]-1,3,5-triazine-2,4-diol + isobutylamine | - |
? | |
3.8.1.8 | 2,4-di(N-secbutylamino)-6-hydroxy-1,3,5-triazine + H2O | - |
Pseudomonas sp. | 6-[(1-methylpropyl)amino]-1,3,5-triazine-2,4-diol + butan-2-amine | - |
? | |
3.8.1.8 | 2,4-di(N-tertbutylamino)-6-hydroxy-1,3,5-triazine + H2O | - |
Pseudomonas sp. | 6-(tert-butylamino)-1,3,5-triazine-2,4-diol + tert-butylamine | - |
? | |
3.8.1.8 | 2,4-diamino-6-hydroxy-1,3,5-triazine + H2O | - |
Pseudomonas sp. | 6-amino-1,3,5-triazine-2,4-diol + NH3 | - |
? | |
3.8.1.8 | 2,4-diethylamino-6-hydroxy-1,3,5-triazine + H2O | - |
Pseudomonas sp. | 6-(methylamino)-1,3,5-triazine-2,4-diol + ethylamine | - |
? | |
3.8.1.8 | 2,4-diisopropylamino-6-hydroxy-1,3,5-triazine + H2O | - |
Pseudomonas sp. | 6-[(1-methylethyl)amino]-1,3,5-triazine-2,4-diol + propan-2-amine | - |
? | |
3.8.1.8 | 2,4-dimethylamino-6-hydroxy-1,3,5-triazine + H2O | - |
Pseudomonas sp. | 6-(methylamino)-1,3,5-triazine-2,4-diol + methylamine | - |
? | |
3.8.1.8 | 2-(N-ethyl-N-methylamino)-4-ethylamino-6-hydroxy-1,3,5-triazine + H2O | - |
Pseudomonas sp. | 6-[ethyl(methyl)amino]-1,3,5-triazine-2,4-diol + ethylamine | - |
? | |
3.8.1.8 | 2-(N-ethylamino)-4-hydroxy-6-(N-hydroxyethylamino)-1,3,5-triazine + H2O | - |
Pseudomonas sp. | 6-[(2-hydroxyethyl)amino]-1,3,5-triazine-2,4-diol + ethylamine | - |
? | |
3.8.1.8 | 2-(N-ethylamino)-4-hydroxy-6-(N-isopropylamino)-1,3,5-triazine + H2O | - |
Pseudomonas sp. | 6-(ethylamino)-1,3,5-triazine-2,4-diol + propan-2-amine | - |
? | |
3.8.1.8 | 2-amino-4-chloro-6-hydroxy-1,3,5-triazine + H2O | - |
Pseudomonas sp. | 6-amino-1,3,5-triazine-2,4-diol + Cl- | - |
? | |
3.8.1.8 | 2-amino-4-hydroxy-6-(N-hydroxyethylamino)-1,3,5-triazine + H2O | - |
Pseudomonas sp. | 6-[(2-hydroxyethyl)amino]-1,3,5-triazine-2,4-diol + NH3 | - |
? | |
3.8.1.8 | 2-amino-4-hydroxy-6-(N-isopropylamino)-1,3,5-triazine + H2O | - |
Pseudomonas sp. | 6-(isopropylamino)-1,3,5-triazine-2,4-diol + NH3 | - |
? | |
3.8.1.8 | 2-amino-6-(N-ethylamino)-4-hydroxy-1,3,5-triazine + H2O | - |
Pseudomonas sp. | 6-(ethylamino)-1,3,5-triazine-2,4-diol + NH3 | - |
? | |
3.8.1.8 | 2-chloro-4-(N-ethylamino)-6-hydroxy-1,3,5-triazine + H2O | - |
Pseudomonas sp. | 6-(ethylamino)-1,3,5-triazine-2,4-diol + Cl- | - |
? | |
3.8.1.8 | 2-chloro-4-hydroxy-6-(N-isopropylamino)-1,3,5-triazine + H2O | - |
Pseudomonas sp. | 6-(isopropylamino)-1,3,5-triazine-2,4-diol + Cl- | - |
? | |
3.8.1.8 | 2-hydroxy-4,6-di(N-hydroxyethylamino)-1,3,5-triazine + H2O | - |
Pseudomonas sp. | 6-[(2-hydroxyethyl)amino]-1,3,5-triazine-2,4-diol + 2-aminoethanol | - |
? | |
3.8.1.8 | 2-hydroxy-4-(N-isopropylamino)-6-(N-(3-methoxypropyl)amino)-1,3,5-triazine + H2O | - |
Pseudomonas sp. | ? | - |
? | |
3.8.1.8 | 6-(ethylamino)-4-(methylamino)-1,3,5-triazin-2-ol + H2O | - |
Pseudomonas sp. | 6-(ethylamino)-1,3,5-triazine-2,4-diol + methylamine | - |
? | |
3.8.1.8 | 6-hydroxy-4-(N-isopropylamino)-2-(N-methylamino)-1,3,5-triazine + H2O | - |
Pseudomonas sp. | 6-[(1-methylethyl)amino]-1,3,5-triazine-2,4-diol + methylamine | - |
? | |
3.8.1.8 | hydroxyatrazine + H2O | - |
Pseudomonas sp. | N-isopropylammelide + ethylamine | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.5.4.43 | dimer | 2 * 52000, recombinant AtzB, SDS-PAGE | Pseudomonas sp. |
3.8.1.8 | dimer | - |
Pseudomonas sp. |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.5.4.43 | AtzB | - |
Pseudomonas sp. |
3.5.4.43 | hydroxyatrazine N-ethylaminohydrolase | - |
Pseudomonas sp. |
3.5.4.43 | More | the enzyme belongs to the amidohydrolase superfamily | Pseudomonas sp. |
3.8.1.8 | AtzB | - |
Pseudomonas sp. |
3.8.1.8 | hydroxyatrazine N-ethylaminohydrolase | - |
Pseudomonas sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.4.43 | 23 | - |
assay at | Pseudomonas sp. |
3.8.1.8 | 23 | - |
activity assay | Pseudomonas sp. |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.8.1.8 | 0.8 | - |
2,4-diisopropylamino-6-hydroxy-1,3,5-triazine | deamination | Pseudomonas sp. | |
3.8.1.8 | 1.4 | - |
6-hydroxy-4-(N-isopropylamino)-2-(N-methylamino)-1,3,5-triazine | deamination | Pseudomonas sp. | |
3.8.1.8 | 1.6 | - |
2-chloro-4-(N-ethylamino)-6-hydroxy-1,3,5-triazine | dechlorination | Pseudomonas sp. | |
3.8.1.8 | 1.9 | - |
2-chloro-4-hydroxy-6-(N-isopropylamino)-1,3,5-triazine | dechlorination | Pseudomonas sp. | |
3.8.1.8 | 3 | - |
hydroxyatrazine | - |
Pseudomonas sp. | |
3.8.1.8 | 3.2 | - |
2-(N-ethylamino)-4-hydroxy-6-(N-isopropylamino)-1,3,5-triazine | deamination | Pseudomonas sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.5.4.43 | 6.5 | 7.5 | - |
Pseudomonas sp. |
3.8.1.8 | 7 | - |
activity assay | Pseudomonas sp. |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.5.4.43 | 6 | 10 | - |
Pseudomonas sp. |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
3.5.4.43 | Pseudomonas sp. | isoelectric focusing, recombinant AtzB | - |
5 |
3.5.4.43 | Pseudomonas sp. | sequence calculation | - |
5.7 |