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Literature summary extracted from

  • Kumasaka, T.; Yamamoto, M.; Furuichi, M.; Nakasako, M.; Teh, A.H.; Kimura, M.; Yamaguchi, I.; Ueki, T.
    Crystal structures of blasticidin S deaminase (BSD): implications for dynamic properties of catalytic zinc (2007), J. Biol. Chem., 282, 37103-37111.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.4.23 expressed in Escherichia coli Aspergillus terreus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.4.23 using 20% (w/v) PEG8000, 50 mM magnesium chloride, and 0.1 M sodium cacodylate at pH 7.0 as precipitant Aspergillus terreus

Protein Variants

EC Number Protein Variants Comment Organism
3.5.4.23 E56Q marginally active Aspergillus terreus
3.5.4.23 H48Q no catalytic activity Aspergillus terreus
3.5.4.23 R90K has a similar structure to the native enzyme except for the tetramer interface region Aspergillus terreus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.4.23 Zn2+ dependent, contains 1 Zn2+ per subunit Aspergillus terreus

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.23 Aspergillus terreus P0C2P0
-
-
3.5.4.23 Aspergillus terreus S-712 / ATCC 28865 P0C2P0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.4.23
-
 Aspergillus terreus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.23 blasticidin S + H2O
-
 Aspergillus terreus deaminohydroxyblasticidin S + NH3
-
 ?
3.5.4.23 blasticidin S + H2O
-
 Aspergillus terreus S-712 / ATCC 28865 deaminohydroxyblasticidin S + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.4.23 homotetramer x-ray crystallography Aspergillus terreus

Synonyms

EC Number Synonyms Comment Organism
3.5.4.23 blasticidin S deaminase
-
 Aspergillus terreus
3.5.4.23 BSD
-
 Aspergillus terreus