EC Number | Cloned (Comment) | Organism |
---|---|---|
1.3.1.9 | expressed in Escherichia coli BL21 Star (DE3) cells | Vibrio cholerae serotype O1 |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.3.1.9 | additional information | the enzyme is very resistant to triclosan | Vibrio cholerae serotype O1 |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.1.9 | 0.053 | - |
NADH | native enzyme, in 0.1 M sodium phosphate buffer (pH 7) | Vibrio cholerae serotype O1 | |
1.3.1.9 | 0.195 | - |
crotonyl-[acyl-carrier protein] | native enzyme, in 0.1 M sodium phosphate buffer (pH 7) | Vibrio cholerae serotype O1 | |
1.3.1.9 | 1.178 | - |
crotonyl-CoA | native enzyme, in 0.1 M sodium phosphate buffer (pH 7) | Vibrio cholerae serotype O1 | |
1.3.1.9 | 3.043 | - |
NADPH | native enzyme, in 0.1 M sodium phosphate buffer (pH 7) | Vibrio cholerae serotype O1 |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.1.9 | Vibrio cholerae serotype O1 | - |
strain ATCC 14547 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.3.1.9 | Ni-NTA column chromatography, Vivapure D maxi H spin column chromatography, and Blue Sepharose column chromatography | Vibrio cholerae serotype O1 |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.1.9 | crotonyl-CoA + NADH + H+ | - |
Vibrio cholerae serotype O1 | butyryl-CoA + NAD+ | - |
? | |
1.3.1.9 | crotonyl-CoA + NADPH + H+ | the native enzyme shows very weak activity with NADPH | Vibrio cholerae serotype O1 | butyryl-CoA + NADP+ | - |
? | |
1.3.1.9 | crotonyl-[acyl-carrier protein] + NADH | - |
Vibrio cholerae serotype O1 | butyryl-[acyl-carrier protein] + NAD+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.3.1.9 | monomer | gel filtration | Vibrio cholerae serotype O1 |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.3.1.9 | enoyl-ACP reductase | - |
Vibrio cholerae serotype O1 |
1.3.1.9 | enoyl-acyl carrier protein reductase | - |
Vibrio cholerae serotype O1 |
1.3.1.9 | FabV | - |
Vibrio cholerae serotype O1 |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.3.1.9 | NADH | shows a very strong preference for NADH over NADPH | Vibrio cholerae serotype O1 |