EC Number | Cloned (Comment) | Organism |
---|---|---|
7.2.2.19 | expression of wild-type and mutant enzymes in HEK-293 cells, expression analysis, overview | Sus scrofa |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
7.2.2.19 | E345D | site-directed mutagenesis, the mutant shows no charge transport pathway, the mutant shows unaltered cell surface expression compared to the wild-type enzyme | Sus scrofa |
7.2.2.19 | E345L | site-directed mutagenesis, the mutant shows no charge transport pathway, the mutant shows unaltered cell surface expression compared to the wild-type enzyme | Sus scrofa |
7.2.2.19 | E345Q | site-directed mutagenesis, the mutant shows an alternative charge transport pathway H3O+-Arg105-Gln161-Gln345, the mutant shows unaltered cell surface expression compared to the wild-type enzyme | Sus scrofa |
7.2.2.19 | K164L | site-directed mutagenesis, the mutant shows an alternative charge transport pathway H3O+-Gln161-Glu345, the mutant shows unaltered cell surface expression compared to the wild-type enzyme | Sus scrofa |
7.2.2.19 | Q161L | site-directed mutagenesis, the mutant shows no charge transport pathway, the mutant shows unaltered cell surface expression compared to the wild-type enzyme | Sus scrofa |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
7.2.2.19 | SCH 28080 | - |
Sus scrofa |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
7.2.2.19 | cell surface | - |
Sus scrofa | 9986 | - |
7.2.2.19 | plasma membrane | - |
Sus scrofa | 5886 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
7.2.2.19 | K+ | activates | Sus scrofa | |
7.2.2.19 | Mg2+ | required | Sus scrofa |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
7.2.2.19 | ATP + H2O + H+/in + K+/out | Sus scrofa | - |
ADP + phosphate + H+/out + K+/in | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
7.2.2.19 | Sus scrofa | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
7.2.2.19 | stomach | gastric membrane | Sus scrofa | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
7.2.2.19 | ATP + H2O + H+/in + K+/out | - |
Sus scrofa | ADP + phosphate + H+/out + K+/in | - |
? | |
7.2.2.19 | ATP + H2O + H+/in + K+/out | protons are charge-transferred from the enzyme's alpha-subunit cytosolic side to H2O in sites 2 and 1, the H2O comes from cytosolic medium, and H3O+ in the sites are transported into lumen during the conformational transition from E1P to E2P, charge transfer pathway charge transfer pathway from hydronium ion in cytosolic medium to Glu345 in cation binding site 2: H3O+-Lys164-Gln161-Glu345 | Sus scrofa | ADP + phosphate + H+/out + K+/in | - |
? | |
7.2.2.19 | ATP + H2O + H+/in + Rb+/out | - |
Sus scrofa | ADP + phosphate + H+/out + Rb+/in | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
7.2.2.19 | More | homology modeling of wild-type and mutant gastric H+,K+-ATPase beta-subunit and molecular dynamics simulation, simulations of charge transfer in wild-type and mutant enzymes, structure comparisons, overview | Sus scrofa |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
7.2.2.19 | gastric H+,K+-ATPase | - |
Sus scrofa |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
7.2.2.19 | 37 | - |
assay at | Sus scrofa |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
7.2.2.19 | 6.8 | - |
assay at | Sus scrofa |