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Literature summary extracted from

  • Fogar, P.; Navaglia, F.; Basso, D.; Greco, E.; Zambon, C.F.; Fadi, E.; Falda, A.; Stranges, A.; Vannozzi, F.; Danesi, R.; Pedrazzoli, S.; Plebani, M.
    Suicide gene therapy with the yeast fusion gene cytosine deaminase/uracil phosphoribosyltransferase is not enough for pancreatic cancer (2007), Pancreas, 35, 224-231.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.4.2.9 medicine the CD/UPRT construct confers 5-fluorocytosine sensitivity to some pancreatic cell lines Saccharomyces cerevisiae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.9 expressed in AsPC-1, BxPC3, CaPan-1, MIA, PaCa-2, Panc-1, and HT-29 cell lines Saccharomyces cerevisiae
3.5.4.1 expression as fusion protein with uracil phosphoribosyltransferase in human pancreatic and colorectal cancer cell lines AsPC1, BxPC3, CaPan1, MIA PaCa2, Panc1, and HT-29, overview Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
3.5.4.1 additional information construction of a fusion protein of yeast cytosine deaminase with yeast uracil phosphoribosyltransferase Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.9 Saccharomyces cerevisiae
-
-
-
3.5.4.1 Saccharomyces cerevisiae Q12178 gene FCY1
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.9 uracil + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Saccharomyces cerevisiae UMP + diphosphate
-
 ?
3.5.4.1 5-fluorocytosine + H2O activation of the prodrug to the cytotoxic drug in cancer therapy, gene therapy with the FCY1 gene, encoding the enzyme, alone is not effective in treatement of pancreatic cancer, co-expression with uracil phosphoribosyltranferase encoding gene FUR1, as fusion enzyme, can confer sensitivity to 5-fluorouracil to some pancreatic cancer cells, but not to all, thus it might also not be an effective therapy in pancreatic cancer, overview Saccharomyces cerevisiae 5-fluorouracil + NH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.2.9 cytosine deaminase/uracil phosphoribosyltransferase bifunctional fusion enzyme Saccharomyces cerevisiae