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Literature summary extracted from

  • Zhu, C.; Warncke, K.
    Reaction of the Co(II)-substrate radical pair catalytic intermediate in coenzyme B12-dependent ethanolamine ammonia-lyase in frozen aqueous solution from 190 to 217 K (2008), Biophys. J., 95, 5890-5900.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.3.1.7 cloning in Escherichia coli overexpression strain incorporating the cloned Salmonella typhimurium EAL coding sequence Salmonella enterica subsp. enterica serovar Typhimurium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.3.1.7 Co2+ included in the cofactor, essential for enzyme binding Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.3.1.7 500000
-
 calculated for the holoenzyme Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.3.1.7 ethanolamine Salmonella enterica subsp. enterica serovar Typhimurium
-
 acetaldehyde + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.3.1.7 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.1.7
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.3.1.7 additional information
-
 enzyme activity is determined by using the coupled assay with alcohol dehydrogenase/NADH, the specific activity of the purified enzyme with aminoethanol as substrate was 20-30 micromol/min/mg Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.1.7 ethanolamine
-
 Salmonella enterica subsp. enterica serovar Typhimurium acetaldehyde + NH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.3.1.7 EAL
-
 Salmonella enterica subsp. enterica serovar Typhimurium
4.3.1.7 ethanolamine ammonia-lyase
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.3.1.7 additional information
-
 additional information the steady-state accumulation, and subsequent cryotrapping, of the Co2-substrate radical pair as the only detectable paramagnetic intermediate, suggest that the step after Co2-substrate radical pair formation is at least partially rate limiting for steady-state turnover Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3.1.7 7.5
-
 assay at Salmonella enterica subsp. enterica serovar Typhimurium

Cofactor

EC Number Cofactor Comment Organism Structure
4.3.1.7 coenzyme B12 adenosylcobalamin Salmonella enterica subsp. enterica serovar Typhimurium