Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Wu, Z.L.; Ethen, C.; Hickey, G.E.; Jiang, W.
    Active 1918 pandemic flu viral neuraminidase has distinct N-glycan profile and is resistant to trypsin digestion (2009), Biochem. Biophys. Res. Commun., 379, 749-753.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.18 expression in SF21 cells influenza A virus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.18 55000
-
 plus dimer and monomer, only the tetramer is enzymatically active. 4 * 55000, SDS-PAGE. Molecular mass is slightly higher than that of monomer and dimer, and only tetramer is resistant to trypsin digestion influenza A virus
3.2.1.18 55000
-
 plus dimer and tetramer, only the tetramer is enzymatically active. Monomer and dimer cannot be oligomerized to the tetramer. 1 * 55000, SDS-PAGE influenza A virus
3.2.1.18 55000
-
 plus monomer and tetramer, only the tetramer is enzymatically active. Monomer and dimer cannot be oligomerized to the tetramer. 2 * 55000, SDS-PAGE influenza A virus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.4 additional information Homo sapiens active 1918 pandemic flu viral neuraminidase is resistant to trypsin digestion ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.18 influenza A virus Q9IGQ6
-
-
3.2.1.18 influenza A virus A/South Carolina/1/1918 H1N1 Q9IGQ6
-
-
3.4.21.4 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.18 glycoprotein deglycosylation of monomer, dimer or tetramer with PNGase F results in molecular masses of 48000 Da. Glycosylation of tetramer is different from monomer and dimer influenza A virus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.18 recombinant protein influenza A virus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.4 additional information active 1918 pandemic flu viral neuraminidase is resistant to trypsin digestion Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.18 dimer plus monomer and tetramer, only the tetramer is enzymatically active. Monomer and dimer cannot be oligomerized to the tetramer. 2 * 55000, SDS-PAGE influenza A virus
3.2.1.18 monomer plus dimer and tetramer, only the tetramer is enzymatically active. Monomer and dimer cannot be oligomerized to the tetramer. 1 * 55000, SDS-PAGE influenza A virus
3.2.1.18 tetramer plus dimer and monomer, only the tetramer is enzymatically active. 4 * 55000, SDS-PAGE. Molecular mass is slightly higher than that of monomer and dimer, and only tetramer is resistant to trypsin digestion influenza A virus