Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Ceh, K.; Demmer, U.; Warkentin, E.; Moll, J.; Thauer, R.K.; Shima, S.; Ermler, U.
    Structural basis of the hydride transfer mechanism in F(420)-dependent methylenetetrahydromethanopterin dehydrogenase (2009), Biochemistry, 48, 10098-10105.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.98.1 overexpression in Escherichia coli Methanopyrus kandleri

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.5.98.1 crystallization trials are performed with the hanging drop vapor diffusion method using a sparse matrix crystallization kit under anaerobic and red light conditions, determination the structures of the Mtd-5,10-methylenetetrahydromethanopterin-, Mtd-5,10-methenyltetrahydromethanopterin- and the Mtd-5,10-methenyltetrahydromethanopterin-F420H2 complexes at 2.1, 2.0, and 1.8 A resolution, both substrate and cofactor bind in a face to face arrangement to an active site cleft, thereby ensuring a direct hydride transfer between their C14a and C5 atoms Methanopyrus kandleri

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.98.1 0.03
-
 coenzyme F420 1 M (NH4)2SO4, at 65°C Methanopyrus kandleri
1.5.98.1 0.04
-
 5,10-methylenetetrahydromethanopterin 1 M (NH4)2SO4, at 65°C Methanopyrus kandleri

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.98.1 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420 Methanopyrus kandleri hydrogenotrophic methanogenesis pathway in class I methanogens, enzyme of the methanogenic energy metabolism that catalyzes the hydride transfer between substrate and product using coenzyme F420 as hydride carrier 5,10-methylenetetrahydromethanopterin + coenzyme F420
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.5.98.1 Methanopyrus kandleri P94951
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.98.1 gel filtration Methanopyrus kandleri

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.98.1 3700
-
 1 M (NH4)2SO4, at 65°C Methanopyrus kandleri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.98.1 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420 hydrogenotrophic methanogenesis pathway in class I methanogens, enzyme of the methanogenic energy metabolism that catalyzes the hydride transfer between substrate and product using coenzyme F420 as hydride carrier Methanopyrus kandleri 5,10-methylenetetrahydromethanopterin + coenzyme F420
-
 r

Subunits

EC Number Subunits Comment Organism
1.5.98.1 hexamer an X-ray structure of methylenetetrahydromethanopterin dehydrogenase at 1.54 A resolution reveals a homohexameric complex organized as a dimer of trimers Methanopyrus kandleri

Synonyms

EC Number Synonyms Comment Organism
1.5.98.1 F420-dependent methylenetetrahydromethanopterin dehydrogenase
-
 Methanopyrus kandleri
1.5.98.1 MTD
-
 Methanopyrus kandleri

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.98.1 coenzyme F420
-
 Methanopyrus kandleri