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Literature summary extracted from

  • Shkil, H.; Stoica, L.; Dmytruk, K.; Smutok, O.; Gonchar, M.; Sibirny, A.; Schuhmann, W.
    Bioelectrochemical detection of L-lactate respiration using genetically modified Hansenula polymorpha yeast cells overexpressing flavocytochrome b2 (2009), Bioelectrochemistry, 76, 175-179.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.2.3 overexpression in Hansenula polymorpha leading to enhanced L-lactate-dependent respiration compared to the wild-type cells Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
1.1.2.3 additional information for detection of the enzyme activity, recombinant enzyme is immobilized by means of a dialysis membrane onto various types of electrode materials in order to investigate the possibility of electrochemically detecting L-lactate respiratio, overview Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.2.3 mitochondrion
-
 Saccharomyces cerevisiae 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.2.3 L-lactate + ferricytochrome c Saccharomyces cerevisiae
-
 pyruvate + ferrocytochrome c + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.2.3 Saccharomyces cerevisiae
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.2.3 3.2
-
 recombinant enzyme in cells of Hansenula polymorpha Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.2.3 L-lactate + ferricytochrome c
-
 Saccharomyces cerevisiae pyruvate + ferrocytochrome c + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.2.3 FC b2
-
 Saccharomyces cerevisiae
1.1.2.3 flavocytochrome b2
-
 Saccharomyces cerevisiae
1.1.2.3 L-lactate:cytochrome c-oxidoreductase
-
 Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.2.3 7.8
-
 assay at Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.2.3 cytochrome c
-
 Saccharomyces cerevisiae