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Literature summary extracted from

  • Colloc'h, N.; Gabison, L.; Monard, G.; Altarsha, M.; Chiadmi, M.; Marassio, G.; Sopkova-de Oliveira Santos, J.; El Hajji, M.; Castro, B.; Abraini, J.H.; Prange, T.
    Oxygen pressurized X-ray crystallography: probing the dioxygen binding site in cofactorless urate oxidase and implications for its catalytic mechanism (2008), Biophys. J., 95, 2415-2422.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.7.3.3 expression in Saccharomyces cerevisiae Aspergillus flavus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.7.3.3 recombinant enzyme in complex with inhibitor 8-azaxanthine in presence of O2 or Cl-, batch technique at room temperature, 10-15 mg/ml protein with an excess of 0.5-2 mg/ml of 8-azaxanthin in 50 mM Tris/HCl, pH 8.5, in the presence of 5-8% w/v PEG 8000 and 0.05 M NaCl, 24-48 h, X-ray diffraction structure determination and analysis at 1.6-1.7 A resolution Aspergillus flavus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.3.3 8-Azaxanthine competitive inhibitor, binding structure, overview Aspergillus flavus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.7.3.3 additional information the catalytic mechanism of UOX does not imply any metal ion Aspergillus flavus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.3.3 urate + O2 + H2O Aspergillus flavus
-
 5-hydroxyisourate + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.7.3.3 Aspergillus flavus Q00511
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.7.3.3 urate + O2 + H2O = 5-hydroxyisourate + H2O2 catalytic mechanism, overview Aspergillus flavus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.3.3 urate + O2 + H2O
-
 Aspergillus flavus 5-hydroxyisourate + H2O2
-
 ?
1.7.3.3 urate + O2 + H2O dioxygen-binding site structure, overview Aspergillus flavus 5-hydroxyisourate + H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.7.3.3 Uox
-
 Aspergillus flavus

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.3.3 additional information the catalytic mechanism of UOX does not imply any cofactor Aspergillus flavus