EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.3.3.15 | 63000 | - |
SDS-PAGE | Roseovarius nubinhibens |
4.1.1.79 | 235000 | - |
native protein with alpha6beta6-structure, determined by gel filtration | Roseovarius nubinhibens |
4.4.1.25 | 35000 | - |
SDS-PAGE | Roseovarius nubinhibens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.3.15 | 2-sulfoacetaldehyde + phosphate | Roseovarius nubinhibens | - |
acetyl phosphate + sulfite | - |
? | |
4.1.1.79 | 3-sulfopyruvate | Roseovarius nubinhibens | - |
2-sulfoacetaldehyde + CO2 | - |
? | |
4.4.1.25 | L-cysteate + H2O | Roseovarius nubinhibens | - |
pyruvate + bisulfite + NH3 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.3.15 | Roseovarius nubinhibens | A3SR25 | - |
- |
4.1.1.79 | Roseovarius nubinhibens | A3SN11 and A3SN10 | subunit alpha and beta | - |
4.4.1.25 | Roseovarius nubinhibens | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.3.15 | by anion exchange chromatography | Roseovarius nubinhibens |
4.1.1.79 | by anion exchange chromatography, hydrophobic interaction chromatography and gel filtration | Roseovarius nubinhibens |
4.4.1.25 | by anion exchange chromatography | Roseovarius nubinhibens |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.3.3.15 | cell culture | ISM strain | Roseovarius nubinhibens | - |
4.1.1.79 | cell culture | ISM strain | Roseovarius nubinhibens | - |
4.4.1.25 | cell culture | ISM strain | Roseovarius nubinhibens | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.3.3.15 | additional information | - |
specific Xsc enzyme activity in extracts from cells grown with cysteate is 0.1 mkat/mg protein | Roseovarius nubinhibens |
2.3.3.15 | additional information | - |
specific Xsc enzyme activity in extracts from cells grown with sulfolactate is 0.9 mkat/mg protein | Roseovarius nubinhibens |
2.3.3.15 | additional information | - |
specific Xsc enzyme activity in extracts from cells grown with taurine is 1.5 mkat/mg protein | Roseovarius nubinhibens |
4.1.1.79 | additional information | - |
specific ComDE enzyme activity in extracts from cells grown with sulfolactate is 0.9 mkat/mg protein, 0 mM Tris-HCl (pH 7.5), 1 mM thiamine diphosphate, 1 to 5 mM sulfopyruvate, and protein (0.1-1.0 mg/ml) | Roseovarius nubinhibens |
4.4.1.25 | additional information | - |
specific CuyA enzyme activity in extracts from cells grown with cysteate is 5.2 mkat/mg protein | Roseovarius nubinhibens |
4.4.1.25 | additional information | - |
specific CuyA enzyme activity in extracts from cells grown with sulfolactate is 1.0 mkat/mg protein | Roseovarius nubinhibens |
EC Number | Storage Stability | Organism |
---|---|---|
4.1.1.79 | purified ComDE is stable under an air atmosphere, it can be stored at 4°C for at least a month without loss of activity | Roseovarius nubinhibens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.3.15 | 2-sulfoacetaldehyde + phosphate | - |
Roseovarius nubinhibens | acetyl phosphate + sulfite | - |
? | |
4.1.1.79 | 3-sulfopyruvate | - |
Roseovarius nubinhibens | 2-sulfoacetaldehyde + CO2 | - |
? | |
4.4.1.25 | L-cysteate + H2O | - |
Roseovarius nubinhibens | pyruvate + bisulfite + NH3 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.1.1.79 | heterodimer | 2 subunits, determined by SDS-PAGE | Roseovarius nubinhibens |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.3.15 | sulfoacetaldehyde acetyltransferase | - |
Roseovarius nubinhibens |
2.3.3.15 | Xsc | - |
Roseovarius nubinhibens |
4.1.1.79 | 3-sulfopyruvate decarboxylase | - |
Roseovarius nubinhibens |
4.1.1.79 | ComDE | - |
Roseovarius nubinhibens |
4.4.1.25 | CuyA | - |
Roseovarius nubinhibens |
4.4.1.25 | cysteate sulfo-lyase | - |
Roseovarius nubinhibens |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.3.3.15 | physiological function | XsC is involved in sulfonate degradation | Roseovarius nubinhibens |
4.1.1.79 | physiological function | ComDE is involved in sulfonate degradation | Roseovarius nubinhibens |
4.4.1.25 | physiological function | CuyA is involved in sulfonate degradation | Roseovarius nubinhibens |