EC Number | Cloned (Comment) | Organism |
---|---|---|
3.4.11.7 | wild type and mutant enzymes are expressed in CHO-K1 cells | Mus musculus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.4.11.7 | T348D | the mutation leads to a modification of the hydrolysis velocity, with no change in the affinity of the recombinant enzymes for the substrate L-Glu-2-naphthylamide, either in the absence or presence of Ca2+ | Mus musculus |
3.4.11.7 | T348S | mutant with increased activity, the mutation leads to a modification of the hydrolysis velocity, with no change in the affinity of the recombinant enzymes for the substrate L-Glu-2-naphthylamide, either in the absence or presence of Ca2+ | Mus musculus |
3.4.11.7 | T348Y | mutant with strongly reduced activity, the mutation leads to a modification of the hydrolysis velocity, with no change in the affinity of the recombinant enzymes for the substrate L-Glu-2-naphthylamide, either in the absence or presence of Ca2+ | Mus musculus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.11.7 | glutamate phosphonate | - |
Mus musculus | |
3.4.11.7 | glutamate thiol | - |
Mus musculus | |
3.4.11.7 | lysine thiol | - |
Mus musculus | |
3.4.11.7 | methionine thiol | - |
Mus musculus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.11.7 | 0.159 | - |
L-Glu-2-naphthylamide | recombinant mutant enzyme T348S, in 50 mM Tris-HCl buffer, pH 7.4, in the presence of 4 mM Ca2+ | Mus musculus | |
3.4.11.7 | 0.198 | - |
L-Glu-2-naphthylamide | recombinant wild type enzyme, in 50 mM Tris-HCl buffer, pH 7.4, in the presence of 4 mM Ca2+ | Mus musculus | |
3.4.11.7 | 0.258 | - |
L-Glu-2-naphthylamide | recombinant mutant enzyme T348D, in 50 mM Tris-HCl buffer, pH 7.4, in the presence of 4 mM Ca2+ | Mus musculus | |
3.4.11.7 | 0.282 | - |
L-Glu-2-naphthylamide | recombinant mutant enzyme T348Y, in 50 mM Tris-HCl buffer, pH 7.4, in the presence of 4 mM Ca2+ | Mus musculus | |
3.4.11.7 | 1.136 | - |
L-Glu-2-naphthylamide | recombinant mutant enzyme T348D, in 50 mM Tris-HCl buffer, pH 7.4, in the absence of Ca2+ | Mus musculus | |
3.4.11.7 | 1.481 | - |
L-Glu-2-naphthylamide | recombinant wild type enzyme, in 50 mM Tris-HCl buffer, pH 7.4, in the absence of Ca2+ | Mus musculus | |
3.4.11.7 | 1.919 | - |
L-Glu-2-naphthylamide | recombinant mutant enzyme T348Y, in 50 mM Tris-HCl buffer, pH 7.4, in the absence of Ca2+ | Mus musculus | |
3.4.11.7 | 2.034 | - |
L-Glu-2-naphthylamide | recombinant mutant enzyme T348S, in 50 mM Tris-HCl buffer, pH 7.4, in the absence of Ca2+ | Mus musculus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.4.11.7 | membrane | - |
Mus musculus | 16020 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.4.11.7 | Ca2+ | the presence of 4 mM Ca2+ enhances the enzymatic activity of wild type enzyme by a factor of 8 | Mus musculus | |
3.4.11.7 | Zn2+ | dependent on | Mus musculus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.4.11.7 | 140000 | - |
minor immature form, SDS-PAGE | Mus musculus |
3.4.11.7 | 160000 | - |
major mature form, SDS-PAGE | Mus musculus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.11.7 | Mus musculus | P16406 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.11.7 | Talon Co2+ column chromatography | Mus musculus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.4.11.7 | brain | - |
Mus musculus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.4.11.7 | 0.25 | - |
mutant enzyme T348Y, in the absence of Ca2+ | Mus musculus |
3.4.11.7 | 0.8 | - |
mutant enzyme T348D, in the absence of Ca2+ | Mus musculus |
3.4.11.7 | 1.6 | - |
mutant enzyme T348D, in the presence of 4 mM Ca2+ | Mus musculus |
3.4.11.7 | 1.98 | - |
wild type enzyme, in the absence of Ca2+ | Mus musculus |
3.4.11.7 | 3.05 | - |
mutant enzyme T348S, in the absence of Ca2+ | Mus musculus |
3.4.11.7 | 7.4 | - |
mutant enzyme T348Y, in the presence of 4 mM Ca2+ | Mus musculus |
3.4.11.7 | 14.67 | - |
wild type enzyme, in the presence of 4 mM Ca2+ | Mus musculus |
3.4.11.7 | 31.54 | - |
mutant enzyme T348S, in the presence of 4 mM Ca2+ | Mus musculus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.11.7 | angiotensin II + H2O | - |
Mus musculus | aspartic acid + angiotensin III | - |
? | |
3.4.11.7 | L-Glu-2-naphthylamide + H2O | - |
Mus musculus | L-Glu + 2-naphthylamine | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.4.11.7 | aminopeptidase A | - |
Mus musculus |
3.4.11.7 | APA | - |
Mus musculus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.11.7 | 2 | 8 | L-Glu-2-naphthylamide | recombinant mutant enzyme T348Y, in 50 mM Tris-HCl buffer, pH 7.4, in the absence of Ca2+ | Mus musculus | |
3.4.11.7 | 40 | - |
L-Glu-2-naphthylamide | recombinant mutant enzyme T348D, in 50 mM Tris-HCl buffer, pH 7.4, in the presence of 4 mM Ca2+ | Mus musculus | |
3.4.11.7 | 92 | - |
L-Glu-2-naphthylamide | recombinant mutant enzyme T348D, in 50 mM Tris-HCl buffer, pH 7.4, in the absence of Ca2+ | Mus musculus | |
3.4.11.7 | 167 | - |
L-Glu-2-naphthylamide | recombinant mutant enzyme T348Y, in 50 mM Tris-HCl buffer, pH 7.4, in the presence of 4 mM Ca2+ | Mus musculus | |
3.4.11.7 | 256 | - |
L-Glu-2-naphthylamide | recombinant wild type enzyme, in 50 mM Tris-HCl buffer, pH 7.4, in the presence of 4 mM Ca2+ | Mus musculus | |
3.4.11.7 | 292 | - |
L-Glu-2-naphthylamide | recombinant wild type enzyme, in 50 mM Tris-HCl buffer, pH 7.4, in the absence of Ca2+ | Mus musculus | |
3.4.11.7 | 575 | - |
L-Glu-2-naphthylamide | recombinant mutant enzyme T348S, in 50 mM Tris-HCl buffer, pH 7.4, in the presence of 4 mM Ca2+ | Mus musculus | |
3.4.11.7 | 640 | - |
L-Glu-2-naphthylamide | recombinant mutant enzyme T348S, in 50 mM Tris-HCl buffer, pH 7.4, in the absence of Ca2+ | Mus musculus |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.11.7 | 0.0001 | - |
lysine thiol | mutant enzyme T348S, in 50 mM Tris-HCl buffer, pH 7.4, in the absence of Ca2+ | Mus musculus | |
3.4.11.7 | 0.00019 | - |
methionine thiol | mutant enzyme T348S, in 50 mM Tris-HCl buffer, pH 7.4, in the absence of Ca2+ | Mus musculus | |
3.4.11.7 | 0.00022 | - |
lysine thiol | wild type enzyme, in 50 mM Tris-HCl buffer, pH 7.4, in the absence of Ca2+ | Mus musculus | |
3.4.11.7 | 0.00026 | - |
methionine thiol | mutant enzyme T348D, in 50 mM Tris-HCl buffer, pH 7.4, in the absence of Ca2+ | Mus musculus | |
3.4.11.7 | 0.00026 | - |
lysine thiol | mutant enzyme T348Y, in 50 mM Tris-HCl buffer, pH 7.4, in the absence of Ca2+ | Mus musculus | |
3.4.11.7 | 0.00029 | - |
methionine thiol | mutant enzyme T348Y, in 50 mM Tris-HCl buffer, pH 7.4, in the absence of Ca2+ | Mus musculus | |
3.4.11.7 | 0.00038 | - |
glutamate thiol | mutant enzyme T348S, in 50 mM Tris-HCl buffer, pH 7.4, in the presence of 4 mM Ca2+ | Mus musculus | |
3.4.11.7 | 0.00058 | - |
methionine thiol | wild type enzyme, in 50 mM Tris-HCl buffer, pH 7.4, in the absence of Ca2+ | Mus musculus | |
3.4.11.7 | 0.00085 | - |
glutamate thiol | mutant enzyme T348Y, in 50 mM Tris-HCl buffer, pH 7.4, in the presence of 4 mM Ca2+ | Mus musculus | |
3.4.11.7 | 0.0009 | - |
lysine thiol | mutant enzyme T348D, in 50 mM Tris-HCl buffer, pH 7.4, in the absence of Ca2+ | Mus musculus | |
3.4.11.7 | 0.00103 | - |
glutamate thiol | mutant enzyme T348D, in 50 mM Tris-HCl buffer, pH 7.4, in the presence of 4 mM Ca2+ | Mus musculus | |
3.4.11.7 | 0.0011 | - |
glutamate thiol | wild type enzyme, in 50 mM Tris-HCl buffer, pH 7.4, in the absence of Ca2+ | Mus musculus | |
3.4.11.7 | 0.00194 | - |
glutamate thiol | mutant enzyme T348S, in 50 mM Tris-HCl buffer, pH 7.4, in the absence of Ca2+ | Mus musculus | |
3.4.11.7 | 0.00197 | - |
glutamate thiol | mutant enzyme T348D, in 50 mM Tris-HCl buffer, pH 7.4, in the absence of Ca2+ | Mus musculus | |
3.4.11.7 | 0.002 | - |
glutamate thiol | wild type enzyme, in 50 mM Tris-HCl buffer, pH 7.4, in the presence of 4 mM Ca2+ | Mus musculus | |
3.4.11.7 | 0.00206 | - |
glutamate thiol | mutant enzyme T348Y, in 50 mM Tris-HCl buffer, pH 7.4, in the absence of Ca2+ | Mus musculus | |
3.4.11.7 | 0.00275 | - |
lysine thiol | mutant enzyme T348S, in 50 mM Tris-HCl buffer, pH 7.4, in the presence of 4 mM Ca2+ | Mus musculus | |
3.4.11.7 | 0.00298 | - |
lysine thiol | wild type enzyme, in 50 mM Tris-HCl buffer, pH 7.4, in the presence of 4 mM Ca2+ | Mus musculus | |
3.4.11.7 | 0.00307 | - |
methionine thiol | mutant enzyme T348Y, in 50 mM Tris-HCl buffer, pH 7.4, in the presence of 4 mM Ca2+ | Mus musculus | |
3.4.11.7 | 0.0036 | - |
lysine thiol | mutant enzyme T348Y, in 50 mM Tris-HCl buffer, pH 7.4, in the presence of 4 mM Ca2+ | Mus musculus | |
3.4.11.7 | 0.00394 | - |
lysine thiol | mutant enzyme T348D, in 50 mM Tris-HCl buffer, pH 7.4, in the presence of 4 mM Ca2+ | Mus musculus | |
3.4.11.7 | 0.00403 | - |
methionine thiol | mutant enzyme T348S, in 50 mM Tris-HCl buffer, pH 7.4, in the presence of 4 mM Ca2+ | Mus musculus | |
3.4.11.7 | 0.00667 | - |
methionine thiol | mutant enzyme T348D, in 50 mM Tris-HCl buffer, pH 7.4, in the presence of 4 mM Ca2+ | Mus musculus | |
3.4.11.7 | 0.0084 | - |
methionine thiol | wild type enzyme, in 50 mM Tris-HCl buffer, pH 7.4, in the presence of 4 mM Ca2+ | Mus musculus |