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Literature summary extracted from

  • Clarke, T.A.; Mills, P.C.; Poock, S.R.; Butt, J.N.; Cheesman, M.R.; Cole, J.A.; Hinton, J.C.; Hemmings, A.M.; Kemp, G.; Soederberg, C.A.; Spiro, S.; Van Wonderen, J.; Richardson, D.J.
    Escherichia coli cytochrome c nitrite reductase NrfA (2008), Methods Enzymol., 437, 63-77.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.7.2.2 purified NrfA, hanging drop vapor diffusion method, five conditions that result in several different crystal packing motifs, e.g. 0.001 ml of protein solution containing 10 mg/ml of NrfA mixed with 0.001 ml of mother liquor containing 100 mM HEPES, pH 7.5, 20% PEG 10000 and incubated in hanging drop trays with a 1 ml well solution at 4°C, 5-14 days, other conditions, overview. X-ray diffraction structure determination and analysis at 1.7-2.5 A resolution Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.2.2 Zn2+ EDTA protects NrfA from zinc inhibition, zinc inhibition kinetics in presence or absence of EDTA, overview Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.7.2.2 periplasm
-
 Escherichia coli
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.7.2.2 Ca2+ active site calcium ion Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.2.2 nitrite + ferrocytochrome c + H+ Escherichia coli the periplasmic cytochrome c nitrite reductase, Nrf, system of Escherichia coli utilizes nitrite as a respiratory electron acceptor by reducing it to ammonium. NO is a proposed intermediate in this six-electron reduction and NrfA can use exogenous NO as a substrate NH3 + H2O + ferricytochrome c
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.7.2.2 Escherichia coli P0ABK9
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.2.2 nitrite + ferrocytochrome c + H+
-
 Escherichia coli NH3 + H2O + ferricytochrome c
-
 ?
1.7.2.2 nitrite + ferrocytochrome c + H+ the periplasmic cytochrome c nitrite reductase, Nrf, system of Escherichia coli utilizes nitrite as a respiratory electron acceptor by reducing it to ammonium. NO is a proposed intermediate in this six-electron reduction and NrfA can use exogenous NO as a substrate Escherichia coli NH3 + H2O + ferricytochrome c
-
 ?
1.7.2.2 nitrite + reduced methyl viologen
-
 Escherichia coli NH3 + H2O + oxidized methyl viologen
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.7.2.2 cytochrome c nitrite reductase
-
 Escherichia coli
1.7.2.2 NrfA
-
 Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.2.2 7
-
 assay at Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.2.2 methyl viologen artificial cofactor, zinc-reduced or dithionite-reduced Escherichia coli