Literature summary extracted from
Ruff, J.; Smits, T.H.; Cook, A.M.; Schleheck, D.
Identification of two vicinal operons for the degradation of 2-aminobenzenesulfonate encoded on plasmid pSAH in Alcaligenes sp. strain O-1 (2010), Microbiol. Res., 165, 288-199.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
1.14.12.14 |
gene absAa, DNA and amino acid sequence determination and analysis. Six genes of the abs cluster are co-transcribed and contain the genes, absAa and absAb, for two subunits of the oxygenase component of the ABS-dioxygenase system. Sequence comparisons, overview |
Alcaligenes sp. O-1 |
1.14.12.14 |
gene absAb, DNA and amino acid sequence determination and analysis. Six genes of the abs cluster are co-transcribed and contain the genes, absAa and absAb, for two subunits of the oxygenase component of the ABS-dioxygenase system. Sequence comparisons, overview |
Alcaligenes sp. O-1 |
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
1.14.12.14 |
2-aminobenzenesulfonate + NADH + H+ + O2 |
Alcaligenes sp. O-1 |
2-aminobenzenesulfonate is used in the manufacture of dyes and pharmaceuticals, and, typical of xenobiotic aryl-sulfonates. The compound is degraded in few native environments, e.g. by Alcaligenes sp. strain O-1, the overall degradative process for ABS in strain O-1 is complex, overview. The pathway shows both split operons and dispersed pathway genes, and no transcriptional regulator |
2,3-dihydroxybenzenesulfonate + NH3 + NAD+ |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.14.12.14 |
Alcaligenes sp. O-1 |
Q9RBG4 |
AbsAb, enzyme component; DSM 6325, six genes of the abs cluster are co-transcribed and contain the genes for two subunits of the oxygenase component of the ABS-dioxygenase system |
- |
1.14.12.14 |
Alcaligenes sp. O-1 |
Q9RBG5 |
AbsAa, enzyme component; DSM 6325, six genes of the abs cluster are co-transcribed and contain the genes for two subunits of the oxygenase component of the ABS-dioxygenase system |
- |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.14.12.14 |
2-aminobenzenesulfonate + NADH + H+ + O2 |
2-aminobenzenesulfonate is used in the manufacture of dyes and pharmaceuticals, and, typical of xenobiotic aryl-sulfonates. The compound is degraded in few native environments, e.g. by Alcaligenes sp. strain O-1, the overall degradative process for ABS in strain O-1 is complex, overview. The pathway shows both split operons and dispersed pathway genes, and no transcriptional regulator |
Alcaligenes sp. O-1 |
2,3-dihydroxybenzenesulfonate + NH3 + NAD+ |
- |
? |
|
1.14.12.14 |
2-aminobenzenesulfonate + NADH + H+ + O2 |
i.e. orthanilate or ABS |
Alcaligenes sp. O-1 |
2,3-dihydroxybenzenesulfonate + NH3 + NAD+ |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
1.14.12.14 |
More |
the enzyme is composed of two components, AbsAa and AbsAb |
Alcaligenes sp. O-1 |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
1.14.12.14 |
ABS-dioxygenase |
- |
Alcaligenes sp. O-1 |
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
1.14.12.14 |
FAD |
part of the reductive component |
Alcaligenes sp. O-1 |
|
1.14.12.14 |
NADH |
- |
Alcaligenes sp. O-1 |
|
General Information
EC Number |
General Information |
Comment |
Organism |
---|
1.14.12.14 |
metabolism |
Alcaligenes sp. O-1 inducibly deaminates 2-aminobenzenesulfonate via dioxygenation to 3-sulfocatechol, which is desulfonated during meta ring-cleavage to yield 2-hydroxymuconate. This intermediate is transformed through the oxalocrotonate-branch of the sulfocatechol meta-pathway, overview. The pathway shows both split operons and dispersed pathway genes, and no transcriptional regulator, overview. The overall degradative process for ABS in strain O-1 is complex, overview |
Alcaligenes sp. O-1 |