Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Winkler, A.; Lyskowski, A.; Riedl, S.; Puhl, M.; Kutchan, T.M.; Macheroux, P.; Gruber, K.
    A concerted mechanism for berberine bridge enzyme (2008), Nat. Chem. Biol., 4, 739-741.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.21.3.3 the crystal structures of berberine bridge enzyme in two different crystal forms, monoclinic and tetragonal, and in complex with (S)-reticuline are determined Eschscholzia californica

Protein Variants

EC Number Protein Variants Comment Organism
1.21.3.3 E417Q mutant, based on structural information, Glu417 essential amino acid for substrate oxidation, bicovalent flavin linkage is not affected by the mutation Eschscholzia californica
1.21.3.3 H459A mutant, based on structural information, His459 do not directly interact with the substrate, bicovalent flavin linkage is not affected by the mutation Eschscholzia californica
1.21.3.3 Y106F mutant, based on structural information, Tyr106 do not directly interact with the substrate, bicovalent flavin linkage is not affected by the mutation Eschscholzia californica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.21.3.3 (S)-reticuline + O2 Eschscholzia californica
-
 (S)-scoulerine + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.21.3.3 Eschscholzia californica
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.21.3.3 (S)-reticuline + O2
-
 Eschscholzia californica (S)-scoulerine + H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.21.3.3 BBE
-
 Eschscholzia californica
1.21.3.3 berberine bridge enzyme
-
 Eschscholzia californica

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.21.3.3 0.054
-
 (S)-reticuline mutant E417Q Eschscholzia californica
1.21.3.3 0.7
-
 (S)-reticuline mutant Y106F Eschscholzia californica
1.21.3.3 3.1
-
 (S)-reticuline mutant H459A Eschscholzia californica
1.21.3.3 8
-
 (S)-reticuline wild-type enzyme Eschscholzia californica

Cofactor

EC Number Cofactor Comment Organism Structure
1.21.3.3 FAD
-
 Eschscholzia californica