EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.14.B11 | D121-AA8 cDNA transformed into yeast cells | Nicotiana tabacum |
1.14.14.B11 | expression in yeast | Nicotiana tabacum |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.14.B11 | 1-aminobenzotriazole | at 1 mM inhibits by 17% | Nicotiana tabacum | |
1.14.14.B11 | Chloropromazine | 1 mM, 87% inhibition of recombinant nicotine demethylase activity in yeast microsomes | Nicotiana tabacum | |
1.14.14.B11 | chlorpromazine | at 1 mM inhibits by 87% | Nicotiana tabacum | |
1.14.14.B11 | CO | partial reverse activity after light exposure | Nicotiana tabacum | |
1.14.14.B11 | cytochrome c | 1 mM, 78% inhibition of recombinant nicotine demethylase activity in yeast microsomes | Nicotiana tabacum | |
1.14.14.B11 | oxidized cytochrome c | at 0.1 mM inhibits by 78% | Nicotiana tabacum | |
1.14.14.B11 | Piperonylbutoxide | at 1 mM inhibits by 42% | Nicotiana tabacum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.14.B11 | 0.0039 | - |
Nicotine | - |
Nicotiana tabacum | |
1.14.14.B11 | 0.0039 | - |
Nicotine | at 25°C, pH 6.25 | Nicotiana tabacum |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.14.14.B11 | membrane | putative N-terminal hydrophobic trans-membrane domain | Nicotiana tabacum | 16020 | - |
1.14.14.B11 | microsome | - |
Nicotiana tabacum | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.14.14.B11 | Iron | cysteine-containing heme-binding region | Nicotiana tabacum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.B11 | nicotine + NADPH + O2 + H+ | Nicotiana tabacum | demethylates nicotine to form nornicotine, a precursor to the nitrosamine N'-nitrosonornicotine | nornicotine + formaldehyde + NADP+ + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.14.B11 | Nicotiana tabacum | Q38Q87 | - |
- |
1.14.14.B11 | Nicotiana tabacum | Q38Q87 | var. 4407 | - |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.14.14.B11 | leaf | - |
Nicotiana tabacum | - |
1.14.14.B11 | leaf | induced in leaves treated with the growth regulator ethylene | Nicotiana tabacum | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.B11 | nicotine + NADPH + O2 + H+ | - |
Nicotiana tabacum | nornicotine + formaldehyde + NADP+ + H2O | - |
? | |
1.14.14.B11 | nicotine + NADPH + O2 + H+ | demethylates nicotine to form nornicotine, a precursor to the nitrosamine N'-nitrosonornicotine | Nicotiana tabacum | nornicotine + formaldehyde + NADP+ + H2O | - |
? | |
1.14.14.B11 | nicotine + NADPH + O2 + H+ | six putative substrate recognition sites | Nicotiana tabacum | nornicotine + formaldehyde + NADP+ + H2O | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.14.B11 | CYP82E4 | - |
Nicotiana tabacum |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.14.B11 | cytochrome P450 | - |
Nicotiana tabacum | |
1.14.14.B11 | NADPH | - |
Nicotiana tabacum | |
1.14.14.B11 | NADPH | is required for full activity | Nicotiana tabacum |
EC Number | Organism | Comment | Expression |
---|---|---|---|
1.14.14.B11 | Nicotiana tabacum | ethylene induces microsomal demethylase activity in leaves | up |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.14.14.B11 | physiological function | only microsomes from yeast expressing the D121-AA8 cDNA exhibit significant nicotine demethylase activity | Nicotiana tabacum |