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Literature summary extracted from

  • Winkler, A.; Puhl, M.; Weber, H.; Kutchan, T.M.; Gruber, K.; Macheroux, P.
    Berberine bridge enzyme catalyzes the six electron oxidation of (S)-reticuline to dehydroscoulerine (2009), Phytochemistry, 70, 1092-1097.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.21.3.3 for expression in Pichia pastoris cells Eschscholzia californica

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.21.3.3 the crystal structure of Berberine bridge enzyme in complex with dehydroscoulerine is determined to 1.63 A resolution Eschscholzia californica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.21.3.3 0.003
-
 (S)-reticuline
-
 Eschscholzia californica
1.21.3.3 0.035
-
 (S)-scoulerine
-
 Eschscholzia californica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.21.3.3 (S)-reticuline + O2 Eschscholzia californica
-
 (S)-scoulerine + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.21.3.3 Eschscholzia californica P30986
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.21.3.3 in a two step purification process Eschscholzia californica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.21.3.3 (S)-reticuline + O2
-
 Eschscholzia californica (S)-scoulerine + H2O2
-
 ?
1.21.3.3 (S)-reticuline + O2 (S)-scoulerine is further oxidized to dehydroscoulerine in a second oxidation step Eschscholzia californica (S)-scoulerine + H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.21.3.3 (S)-reticuline oxidase
-
 Eschscholzia californica
1.21.3.3 BBE
-
 Eschscholzia californica
1.21.3.3 berberine bridge enzyme
-
 Eschscholzia californica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.21.3.3 37
-
 activity assay Eschscholzia californica

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.21.3.3 0.0025
-
 (S)-scoulerine
-
 Eschscholzia californica
1.21.3.3 8
-
 (S)-reticuline
-
 Eschscholzia californica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.21.3.3 9
-
 activity assay Eschscholzia californica

Cofactor

EC Number Cofactor Comment Organism Structure
1.21.3.3 FAD
-
 Eschscholzia californica