Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Pozzuolo, S.; Breme, U.; Salis, B.; Taylor, G.; Tonon, G.; Orsini, G.
    Efficient bacterial expression of fusion proteins and their selective processing by a recombinant Kex-1 protease (2008), Protein Expr. Purif., 59, 334-341.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.4.16.6 biotechnology Kex-1-C611 is proposed as a convenient biotechnological reagent for the cleavage of fusion proteins Kluyveromyces lactis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.16.6 a truncated form of Kex-1, Kex-1-C611, is constructed comprising the amino acid residues from 104-611 and lacks the transmembrane domain Kluyveromyces lactis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.16.6 Ca2+
-
 Kluyveromyces lactis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.16.6 58000
-
 calculated, truncated Kex-1-C611 Kluyveromyces lactis
3.4.16.6 67000
-
 determined by SDS-PAGE, truncated Kex-1-C611 Kluyveromyces lactis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.16.6 additional information Kluyveromyces lactis preferential release of a C-terminal arginine or lysine residue ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.16.6 Kluyveromyces lactis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.16.6 archieved by a two-step chromatographic process using cation-exchange, SP Sepharose, followed by anion-exchange, DEAE Sepharose FF resin Kluyveromyces lactis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.16.6 additional information
-
 60 U/ml, purified truncated Kex-1-C611 Kluyveromyces lactis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.16.6 additional information preferential release of a C-terminal arginine or lysine residue Kluyveromyces lactis ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.16.6 Kex-1 endopeptidase
-
 Kluyveromyces lactis
3.4.16.6 Kex-1 protease
-
 Kluyveromyces lactis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.16.6 25
-
 cleavage assay Kluyveromyces lactis