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Literature summary extracted from

  • Gay, S.C.; Fribourgh, J.L.; Donohoue, P.D.; Segel, I.H.; Fisher, A.J.
    Kinetic properties of ATP sulfurylase and APS kinase from Thiobacillus denitrificans (2009), Arch. Biochem. Biophys., 489, 110-117.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.4 His-tag version expressed in Escherichia coli BL21-DE3 Thiobacillus denitrificans ATCC 25259

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.4 0.0045
-
adenylyl sulfate ATP synthesis Thiobacillus denitrificans ATCC 25259
2.7.7.4 0.029
-
diphosphate ATP synthesis Thiobacillus denitrificans ATCC 25259
2.7.7.4 0.78
-
MgATP2- adenylyl sulfate synthesis Thiobacillus denitrificans ATCC 25259
2.7.7.4 17
-
sulfate adenylyl sulfate synthesis Thiobacillus denitrificans ATCC 25259

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.4 Thiobacillus denitrificans ATCC 25259 Q3SEZ6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.4 immobilized metal ion affinity chromatography Thiobacillus denitrificans ATCC 25259

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.4 31.5
-
adenylyl sulfate synthesis Thiobacillus denitrificans ATCC 25259
2.7.7.4 352
-
ATP synthesis Thiobacillus denitrificans ATCC 25259

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.4 diphosphate + adenylyl sulfate
-
Thiobacillus denitrificans ATCC 25259 ATP + sulfate
-
r
2.7.7.4 MgATP2- + sulfate
-
Thiobacillus denitrificans ATCC 25259 Mg-diphosphate + adenylyl sulfate
-
r

Synonyms

EC Number Synonyms Comment Organism
2.7.7.4 ATP sulfurylase
-
Thiobacillus denitrificans ATCC 25259

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.7.4 23.2
-
sulfate adenylyl sulfate synthesis Thiobacillus denitrificans ATCC 25259
2.7.7.4 260
-
adenylyl sulfate ATP synthesis Thiobacillus denitrificans ATCC 25259