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Literature summary extracted from
- Identification, functional characterization and developmental regulation of sesquiterpene synthases from sunflower capitate glandular trichomes (2009), BMC Plant Biol., 9, 86.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.2.3.13 | expressed in Saccharomyces cerevisiae strain EPY300 as a thioredoxin-fusion protein | Helianthus annuus |
| 4.2.3.13 | thioredoxin-fusion protein expressed in Escherichia coli, expressed in Saccharomyces cerevisiae EPY300 | Helianthus annuus |
| 4.2.3.23 | thioredoxin-fusion protein expressed in Escherichia coli | Helianthus annuus |
| 4.2.3.133 | expressed in Saccharomyces cerevisiae strain EPY300 as a thioredoxin-fusion protein | Helianthus annuus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.3.23 | farnesyl diphosphate | substrate inhibition above 0.0025 mM | Helianthus annuus |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.3.23 | 0.00074 | - |
(2E,6E)-farnesyl diphosphate | - |
Helianthus annuus | |
| 4.2.3.23 | 0.00082 | - |
(2E,6E)-farnesyl diphosphate | - |
Helianthus annuus | |
| 4.2.3.23 | 0.00106 | - |
(2E,6E)-farnesyl diphosphate | thioredoxin-fusion protein | Helianthus annuus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.2.3.13 | 64200 | - |
x * 64200, calculated | Helianthus annuus |
| 4.2.3.13 | 64200 | - |
x * 64200, calculated from amino acid sequence | Helianthus annuus |
| 4.2.3.23 | 64200 | - |
x * 64200, calculated | Helianthus annuus |
| 4.2.3.23 | 64400 | - |
x * 64400, calculated | Helianthus annuus |
| 4.2.3.133 | 64200 | - |
x * 64200, calculated from amino acid sequence | Helianthus annuus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.3.13 | Helianthus annuus | Q4U3F6 | - |
- |
| 4.2.3.13 | Helianthus annuus | Q4U3F6 | isoform TPS2; cultivar HA300 | - |
| 4.2.3.23 | Helianthus annuus | B0FGA9 | - |
- |
| 4.2.3.23 | Helianthus annuus | Q4U3F7 | - |
- |
| 4.2.3.133 | Helianthus annuus | Q4U3F6 | isoform TPS2; cultivar HA300 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.2.3.13 | Ni-NTA affinity chromatography | Helianthus annuus |
| 4.2.3.13 | Ni-NTA affinity column chromatography | Helianthus annuus |
| 4.2.3.23 | Ni-NTA affinity chromatography | Helianthus annuus |
| 4.2.3.133 | Ni-NTA affinity column chromatography | Helianthus annuus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 4.2.3.13 | glandular trichome | - |
Helianthus annuus | - |
| 4.2.3.13 | leaf | only young leaf | Helianthus annuus | - |
| 4.2.3.13 | trichome | - |
Helianthus annuus | - |
| 4.2.3.23 | root | - |
Helianthus annuus | - |
| 4.2.3.23 | trichome | - |
Helianthus annuus | - |
| 4.2.3.133 | glandular trichome | - |
Helianthus annuus | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.3.13 | (2E,6E)-farnesyl diphosphate | - |
Helianthus annuus | delta-cadinene + diphosphate | delta-cadinene is one of the two main products, while alpha-muuorlene, beta-caryophyllene, alpha-humulene and alpha-copaene are minor products | ? | |
| 4.2.3.13 | additional information | - |
Helianthus annuus | ? | products (in vivo expression in Saccharomyces cerevisiae): delta-cadinene and a compound with a cadinene skeleton, minor products: alpha-muurolene, beta-caryophyllene, alpha-humulene and alpha-copaene | ? | |
| 4.2.3.23 | (2E,6E)-farnesyl diphosphate | - |
Helianthus annuus | germacrene A + diphosphate | - |
? | |
| 4.2.3.133 | (2E,6E)-farnesyl diphosphate | - |
Helianthus annuus | alpha-copaene + diphosphate | delta-cadinene is one of the two main products, while alpha-muuorlene, beta-caryophyllene, alpha-humulene and alpha-copaene are minor products | ? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.2.3.13 | ? | x * 64200, calculated | Helianthus annuus |
| 4.2.3.13 | ? | x * 64200, calculated from amino acid sequence | Helianthus annuus |
| 4.2.3.23 | ? | x * 64200, calculated | Helianthus annuus |
| 4.2.3.23 | ? | x * 64400, calculated | Helianthus annuus |
| 4.2.3.133 | ? | x * 64200, calculated from amino acid sequence | Helianthus annuus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.2.3.13 | cadinene synthase | - |
Helianthus annuus |
| 4.2.3.13 | sesquiterpene synthase | - |
Helianthus annuus |
| 4.2.3.13 | TPS2 | isoform | Helianthus annuus |
| 4.2.3.23 | germacrene A synthase 1 | - |
Helianthus annuus |
| 4.2.3.23 | germacrene A synthase 2 | - |
Helianthus annuus |
| 4.2.3.133 | sesquiterpene synthase | - |
Helianthus annuus |
| 4.2.3.133 | TPS2 | isoform | Helianthus annuus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.3.23 | 7.5 | - |
- |
Helianthus annuus |
| 4.2.3.23 | 7.7 | - |
- |
Helianthus annuus |
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