EC Number | Application | Comment | Organism |
---|---|---|---|
1.13.11.12 | additional information | the manipulating of the expression of the LOX gene may provide a basis for advancing both the fundamental and applied biology of rice | Oryza sativa |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.13.11.B6 | expression in Escherichia coli | Oryza sativa |
1.13.11.12 | LOX1 ORF cloned into vector pET30a(+) for overexpression in Escherichia coli. LOX1-green GFP fusion construct, under the control of the cauliflower mosaic virus 35S promoter, expressed in Arabidopsis plants. Transgenic rice lines carrying either sense or antisense constructs under the control of a cauliflower mosaic virus 35S promoter | Oryza sativa |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.13.11.12 | chloroplast | - |
Oryza sativa | 9507 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.13.11.B6 | 105000 | - |
1 * 105000, recombinant enzyme including His-tag, SDS-PAGE | Oryza sativa |
1.13.11.12 | 105000 | - |
1 * 105000, SDS-PAGE, His-tagged enzyme | Oryza sativa |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.11.B6 | linoleate + O2 | Oryza sativa | - |
(9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate + (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoate | the ratio of (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoate to (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate is 4:3 | ? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.13.11.B6 | Oryza sativa | Q27PX2 | subsp. japonica, variety Beilu PL2 | - |
1.13.11.12 | Oryza sativa | Q27PX2 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.13.11.12 | soluble LOX purified with His bind kits | Oryza sativa |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.13.11.B6 | seed | OsLOX1 transcripts are detected at low abundance in immature seeds and newly germinated seedlings, but accumulate rapidly and transiently in response to wounding or brown planthopper attack, reaching a peak 3 h after wounding and 6 h after insect feeding | Oryza sativa | - |
1.13.11.B6 | seedling | OsLOX1 transcripts are detected at low abundance in immature seeds and newly germinated seedlings, but accumulate rapidly and transiently in response to wounding or brown planthopper attack, reaching a peak 3 h after wounding and 6 h after insect feeding | Oryza sativa | - |
1.13.11.12 | seed | low abundance in immature seeds | Oryza sativa | - |
1.13.11.12 | seedling | low abundance in newly germinated seedlings | Oryza sativa | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.11.B6 | linoleate + O2 | - |
Oryza sativa | (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate + (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoate | the ratio of (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoate to (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate is 4:3 | ? | |
1.13.11.B6 | linoleate + O2 | - |
Oryza sativa | (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate + (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoate | dual positional specificity. The ratio of (10E,12Z)-9-hydroperoxy-10,12-octadecadienoate to (9Z,11E)-13-hydroperoxy-9,11-octadecadienoate is 4:3. Both products are predominantly in the S configuration | ? | |
1.13.11.12 | linoleate + O2 | - |
Oryza sativa | (9Z,11E)-(13S)-13-hydroperoxyoctadeca-9,11-dienoate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.13.11.B6 | monomer | 1 * 105000, recombinant enzyme including His-tag, SDS-PAGE | Oryza sativa |
1.13.11.12 | monomer | 1 * 105000, SDS-PAGE, His-tagged enzyme | Oryza sativa |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.13.11.B6 | OsLOX1 | - |
Oryza sativa |
1.13.11.12 | linoleate:oxygen oxidoreductase | - |
Oryza sativa |
1.13.11.12 | LOX1 | - |
Oryza sativa |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.13.11.B6 | 4.8 | - |
- |
Oryza sativa |
1.13.11.12 | 4.8 | - |
- |
Oryza sativa |
EC Number | Organism | Comment | Expression |
---|---|---|---|
1.13.11.B6 | Oryza sativa | transformants with a lower level of OsLOX1 expression are less able to tolerate brown planthopper attack | down |
1.13.11.B6 | Oryza sativa | transformants with enhanced level of OsLOX1 expression are more resistant to brown planthopper attack | up |
1.13.11.12 | Oryza sativa | in all antisense transgenic rice lines and more than half of the sense lines the expression levels of LOX1, the levels of enzyme activity, and the levels of the endogenous LOX1 products (jasmonic acid, (Z)-3-hexenal and colneleic acid) at 6, 48, and 48 h after brown planthopper feeding respectively, are below the levels found in non-transgenic control plants | down |
1.13.11.12 | Oryza sativa | LOX1 transcripts accumulate rapidly and transiently in response to wounding or brown planthopper attack, reaching a peak 3 h after wounding and 6 h after insect feeding. Transgenic rice lines show altered LOX1 activity | up |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.13.11.B6 | physiological function | OsLOX1 is likely to be the main LOX involved in rice response to biotic and wounding stress. 13-LOX pathway products (such as (Z)-3-hexenyl acetate) protect plants against herbivores by inducing the production of several defence molecules and by attracting herbivore predators. products of 9-LOXs might participate in plant resistance to insect attack, as well | Oryza sativa |
1.13.11.12 | physiological function | LOX1 is a non-conventional LOX with 13- or dual position-specific LOX activity. LOX1 is involved in the synthesis of jasmonic acid, colneleic acid, and (Z)-3-hexenal. The LOX1 product is involved in tolerance of the rice plant to wounding and brown planthopper Nilaparvata lugens attack | Oryza sativa |