EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.313 | 4-oxobutyrate | 90% substrate inhibition at 20 mM | Chromohalobacter salexigens | |
1.1.1.313 | additional information | not inhibited by formaldehyde, acetaldehyde, betaine aldehyde, propionaldehyde, DL-glyceraldehyde, phosphonoacetaldehyde, glyoxylate, 2-oxobutyrate, 4-oxobutyrate and 3-sulfopropanaldehyde | Chromohalobacter salexigens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.313 | 0.061 | - |
NADPH | in 0.02 mMTris/H2SO4 buffer, pH 9.0, at 23°C | Chromohalobacter salexigens | |
1.1.1.313 | 0.13 | - |
2-sulfoacetaldehyde | in 0.02 mMTris/H2SO4 buffer, pH 9.0, at 23°C | Chromohalobacter salexigens |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.313 | 27000 | - |
SDS-PAGE | Chromohalobacter salexigens |
1.1.1.313 | 27100 | - |
estimated from amino acid sequence | Chromohalobacter salexigens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.313 | Chromohalobacter salexigens | - |
- |
- |
1.1.1.313 | Chromohalobacter salexigens DSM 3043 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.313 | Mono Q column chromatography, phenyl Superose gel filtration | Chromohalobacter salexigens |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.1.1.313 | culture condition:ammonium-grown cell | IsfD is detected at low levels in extracts of ammonium-grown cells | Chromohalobacter salexigens | - |
1.1.1.313 | culture condition:taurine-grown cell | IsfD is detected at high levels in extracts of taurine-grown cells | Chromohalobacter salexigens | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.313 | 2-sulfoacetaldehyde + NADPH + H+ | - |
Chromohalobacter salexigens | isethionate + NADP+ | - |
ir | |
1.1.1.313 | 2-sulfoacetaldehyde + NADPH + H+ | - |
Chromohalobacter salexigens DSM 3043 | isethionate + NADP+ | - |
ir | |
1.1.1.313 | 4-oxobutyrate + NADPH + H+ | - |
Chromohalobacter salexigens | ? | - |
ir | |
1.1.1.313 | 4-oxobutyrate + NADPH + H+ | - |
Chromohalobacter salexigens DSM 3043 | ? | - |
ir | |
1.1.1.313 | additional information | NADH is not a substrate | Chromohalobacter salexigens | ? | - |
? | |
1.1.1.313 | additional information | NADH is not a substrate | Chromohalobacter salexigens DSM 3043 | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.313 | isethionate formation, reductase D | - |
Chromohalobacter salexigens |
1.1.1.313 | IsfD | isoform, this enzyme represents the major portion of the IsfD activity described for taurine-grown cells | Chromohalobacter salexigens |
1.1.1.313 | IsfD2 | isoform, this enzyme is responsible for the low activity observed in extracts of ammonium-grown cells | Chromohalobacter salexigens |
1.1.1.313 | NADPH-dependent sulfoacetaldehyde reductase | - |
Chromohalobacter salexigens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.313 | 9 | - |
in Tris/H2SO4 buffer | Chromohalobacter salexigens |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.313 | 6.5 | 9 | IsfD shows a broad pH optimum from about pH 6.5 to pH 9.5. There is a steep increase in activity from pH 4.5 (negligible activity) and an equally steep decrease above pH 9.5 | Chromohalobacter salexigens |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.313 | 9 | - |
IsfD is stable in Tris/H2SO4 buffer, pH 9.0 | Chromohalobacter salexigens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.313 | NADPH | highly specific for NADPH. NADH is not a substrate. | Chromohalobacter salexigens |