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Literature summary extracted from

  • Casutt, M.S.; Wendelspiess, S.; Steuber, J.; Fritz, G.
    Crystallization of the Na+-translocating NADH:quinone oxidoreductase from Vibrio cholerae (2010), Acta Crystallogr. Sect. F, 66, 1677-1679.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.2.1.1 hexahistidine-tagged Na+-NQR is expressed in Vibrio cholerae strain DELTAnqr lacking expression of the NQR complex Vibrio cholerae serotype O1

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
7.2.1.1 sitting drop vapor diffusion method, using 40 mM KSCN, 21.0% PEG 2000 MME, 100 mM Tris acetate pH 8.5 and 8% (v/v) 1-propanol, cofactors such as FAD, riboflavin or NADH are not added during crystallization Vibrio cholerae serotype O1

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.2.1.1 membrane
-
 Vibrio cholerae serotype O1 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.2.1.1 NADH + H+ + ubiquinone + n Na+/in Vibrio cholerae serotype O1
-
 NAD+ + ubiquinol + n Na+/out
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
7.2.1.1 Vibrio cholerae serotype O1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.2.1.1 Ni-NTA agarose column chromatography and Superdex 200 gel filtration Vibrio cholerae serotype O1

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.2.1.1 NADH + H+ + ubiquinone + n Na+/in
-
 Vibrio cholerae serotype O1 NAD+ + ubiquinol + n Na+/out
-
 ?

Subunits

EC Number Subunits Comment Organism
7.2.1.1 heterohexamer Na+-NQR is a heterohexameric complex consisting of six different protein subunits (NqrA-NqrF) Vibrio cholerae serotype O1

Synonyms

EC Number Synonyms Comment Organism
7.2.1.1 Na+-NQR
-
 Vibrio cholerae serotype O1
7.2.1.1 Na+-translocating NADH:quinone oxidoreductase
-
 Vibrio cholerae serotype O1
7.2.1.1 sodium-translocating NADH:quinone oxidoreductase
-
 Vibrio cholerae serotype O1

Cofactor

EC Number Cofactor Comment Organism Structure
7.2.1.1 FAD noncovalently bound Vibrio cholerae serotype O1
7.2.1.1 FMN two covalently bound FMNs Vibrio cholerae serotype O1
7.2.1.1 NADH
-
 Vibrio cholerae serotype O1
7.2.1.1 riboflavin noncovalently bound Vibrio cholerae serotype O1
7.2.1.1 ubiquinone-8
-
 Vibrio cholerae serotype O1
7.2.1.1 [2Fe-2S]-center
-
 Vibrio cholerae serotype O1