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Literature summary extracted from

  • Kim, B.; Sullivan, R.P.; Zhao, H.
    Cloning, characterization, and engineering of fungal L-arabinitol dehydrogenases (2010), Appl. Microbiol. Biotechnol., 87, 1407-1414.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.12 D212S/I213R no activity with NAD+, slight activity with NADP+ Penicillium chrysogenum
1.1.1.12 D213S/I214R significantly higher preference for NADP+ over NAD+ Aspergillus niger
1.1.1.12 D224S/I225R significantly altered cofactor specificity from NAD+ to NADP+ Trichoderma reesei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.12 0.2
-
 NAD+ pH 7.0, 22°C Aspergillus niger
1.1.1.12 0.2
-
 NAD+ pH 7.0, 22°C Trichoderma reesei
1.1.1.12 0.3
-
 NAD+ pH 7.0, 22°C Penicillium chrysogenum
1.1.1.12 18
-
 L-arabinitol pH 7.0, 22°C Trichoderma reesei
1.1.1.12 25
-
 L-arabinitol pH 7.0, 22°C Aspergillus niger
1.1.1.12 37
-
 L-arabinitol pH 7.0, 22°C Penicillium chrysogenum

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.12 Aspergillus niger A2QAC0
-
-
1.1.1.12 Penicillium chrysogenum B6HI95 strain ATCC 28089 /DSM 1075 /Wisconsin 54-1255
-
1.1.1.12 Penicillium chrysogenum Wisconsin 54-1255 B6HI95 strain ATCC 28089 /DSM 1075 /Wisconsin 54-1255
-
1.1.1.12 Trichoderma reesei Q96V44
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.12 8.7
-
 pH 7.0, 22°C Trichoderma reesei
1.1.1.12 11.7
-
 pH 7.0, 22°C Aspergillus niger
1.1.1.12 25.3
-
 pH 7.0, 22°C Penicillium chrysogenum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.12 L-arabinitol + NAD+
-
 Aspergillus niger L-xylulose + NADH + H+
-
 ?
1.1.1.12 L-arabinitol + NAD+
-
 Trichoderma reesei L-xylulose + NADH + H+
-
 ?
1.1.1.12 L-arabinitol + NAD+
-
 Penicillium chrysogenum L-xylulose + NADH
-
 ?
1.1.1.12 L-arabinitol + NAD+
-
 Penicillium chrysogenum Wisconsin 54-1255 L-xylulose + NADH
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.12 45
-
-
 Aspergillus niger
1.1.1.12 45
-
-
 Trichoderma reesei
1.1.1.12 45
-
-
 Penicillium chrysogenum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.12 5.76
-
 L-arabinitol pH 7.0, 22°C Trichoderma reesei
1.1.1.12 7.26
-
 NAD+ pH 7.0, 22°C Trichoderma reesei
1.1.1.12 8.23
-
 NAD+ pH 7.0, 22°C Aspergillus niger
1.1.1.12 8.45
-
 L-arabinitol pH 7.0, 22°C Aspergillus niger
1.1.1.12 17.3
-
 NAD+ pH 7.0, 22°C Penicillium chrysogenum
1.1.1.12 18.1
-
 L-arabinitol pH 7.0, 22°C Penicillium chrysogenum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.12 additional information no activity with NADP+ Aspergillus niger
1.1.1.12 additional information no activity with NADP+ Trichoderma reesei
1.1.1.12 additional information no activity with NADP+ Penicillium chrysogenum
1.1.1.12 NAD+
-
 Aspergillus niger
1.1.1.12 NAD+
-
 Trichoderma reesei
1.1.1.12 NAD+
-
 Penicillium chrysogenum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.12 0.32
-
 L-arabinitol pH 7.0, 22°C Trichoderma reesei
1.1.1.12 0.32
-
 L-arabinitol pH 7.0, 22°C Penicillium chrysogenum
1.1.1.12 0.338
-
 L-arabinitol pH 7.0, 22°C Aspergillus niger
1.1.1.12 41.17
-
 NAD+ pH 7.0, 22°C Aspergillus niger
1.1.1.12 44.8
-
 NAD+ pH 7.0, 22°C Trichoderma reesei
1.1.1.12 57.7
-
 NAD+ pH 7.0, 22°C Penicillium chrysogenum