EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.9 | expression in Escherichia coli | Rhizobium etli |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.1.1.9 | multi-template homology modeling. The structural model of XDH obtained consists of a classical alpha/beta Rossmann fold pattern commonly found in the MDR family, which is organized into two beta-barrel domains, the coenzyme-binding residues 163-300 and catalytic residues 1-162, 301-364 | Rhizobium etli |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.9 | EDTA | 1-10 mM, strong inhibition | Rhizobium etli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.9 | 0.25 | - |
NAD+ | pH 9.5, 25°C | Rhizobium etli | |
1.1.1.9 | 12.1 | - |
xylitol | pH 9.5, 25°C | Rhizobium etli |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.9 | Ca2+ | 1 mM, 18% of the activity with Mg2+ | Rhizobium etli | |
1.1.1.9 | Co2+ | 1 mM, 38% of the activity with Mg2+ | Rhizobium etli | |
1.1.1.9 | Cu2+ | 1 mM, 51% of the activity with Mg2+ | Rhizobium etli | |
1.1.1.9 | Mg2+ | or Mn2+, required | Rhizobium etli | |
1.1.1.9 | Mn2+ | or Mg2+, required. At 1 mM, 108% of the activity with Mg2+ | Rhizobium etli | |
1.1.1.9 | additional information | activity is not significantly stimulated by Ba2+, Ca2+, Fe2+, Hg2+, or K+ | Rhizobium etli | |
1.1.1.9 | Zn2+ | 1 mM, 62% of the activity with Mg2+. The deduced XDH gene product possesses the Zn2+-containing ADH signature sequence [GHE]xx[G]xxxxx[G]xx[V] and the catalytic Zn2+-binding site Cys41, His66, Glu67, and Glu152 | Rhizobium etli |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.9 | 34000 | - |
4 * 35858, calculated, 4 * 34000, SDS-PAGE | Rhizobium etli |
1.1.1.9 | 35858 | - |
4 * 35858, calculated, 4 * 34000, SDS-PAGE | Rhizobium etli |
1.1.1.9 | 135000 | - |
gel filtration | Rhizobium etli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.9 | Rhizobium etli | Q2K0Q7 | strain CFN42 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.9 | recombinant enzmye | Rhizobium etli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.9 | D-mannitol + NAD+ | 8% of the activity with xylitol | Rhizobium etli | ? + NADH + H+ | - |
? | |
1.1.1.9 | D-sorbitol + NAD+ | 90% of the activity with xylitol | Rhizobium etli | D-fructose + NADH + H+ | - |
? | |
1.1.1.9 | erythritol + NAD+ | 0.77% of the activity with xylitol | Rhizobium etli | ? + NADH + H+ | - |
? | |
1.1.1.9 | galactitol + NAD+ | 0.98% of the activity with xylitol | Rhizobium etli | ? + NADH + H+ | - |
? | |
1.1.1.9 | L-arabinitol + NAD+ | 21% of the activity with xylitol | Rhizobium etli | ? + NADH + H+ | - |
? | |
1.1.1.9 | L-threitol + NAD+ | 0.05% of the activity with xylitol | Rhizobium etli | ? + NADH + H+ | - |
? | |
1.1.1.9 | L-threitol + NAD+ | 75% of the activity with xylitol | Rhizobium etli | ? + NADH + H+ | - |
? | |
1.1.1.9 | ribitol + NAD+ | 40% of the activity with xylitol | Rhizobium etli | ? + NADH + H+ | - |
? | |
1.1.1.9 | xylitol + NAD+ | - |
Rhizobium etli | D-xylulose + NADH + H+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.9 | tetramer | 4 * 35858, calculated, 4 * 34000, SDS-PAGE | Rhizobium etli |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.9 | 70 | - |
- |
Rhizobium etli |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.9 | 40 | - |
half-life 120 min | Rhizobium etli |
1.1.1.9 | 53.1 | - |
half denaturation temperature | Rhizobium etli |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.9 | 9.5 | - |
- |
Rhizobium etli |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.9 | additional information | activity with NADP+ is 4% of the activity with NAD+ | Rhizobium etli | |
1.1.1.9 | NAD+ | the deduced XDH gene product possesses an NAD-binding glycine-rich Rossmann fold domain [GXGXXG] present in MDR superfamily | Rhizobium etli |