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Literature summary extracted from

  • Lu, Q.; McAlister-Henn, L.
    Peroxisomal localization and function of NADP+-specific isocitrate dehydrogenases in yeast (2010), Arch. Biochem. Biophys., 493, 125-134.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.42 expression of His-tagged IDPA in Escherichia coli strain BL21(DE3), expression of IDPA inm Saccharomyces cerevisiae, it behaves similar to the yeast IDP2 harboring a type I peroxisomal targeting sequenceand occurs in cytosol and peroxisomes, complementation study in Saccharomyces cerevisiae disruption mutants expressing the full-length IDPA enzyme from Aspergillus nidulans, and IDPA lacking the mitochondrial targeting sequence and containing a different PTS1, which results in the same expression level and subcellular orientation, subcellular localization study, overview Aspergillus nidulans

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.42 additional information construction of IDP1, IDP2, and IDP3 disruption mutants, and of double and triple disruption mutants in haploid strain MMY011. Complementation study in disruption mutants expressing the full-length IDPA enzyme from Aspergillus nidulans, which behaves similar to the yeast IDP2 harboring a type I peroxisomal targeting sequence, PTS1, and occurs in cytosol and peroxisomes, subcellular localization study, overview. Expression of IDPA lacking the mitochondrial targeting sequence and containing a different PTS1 results in the same expression level and subcellular orientation Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.42 cytosol
-
 Aspergillus nidulans 5829
-
1.1.1.42 cytosol IDP2 carrying a type I peroxisomal targeting sequence, IDP2+CKL, is only partially localized to peroxisomes, the enzyme is able to function either instead of peroxisomal IDP3 or as cytosolic IDP2 Saccharomyces cerevisiae 5829
-
1.1.1.42 mitochondrion IDP1 Saccharomyces cerevisiae 5739
-
1.1.1.42 additional information subcellular localization study of isozymes, overview Saccharomyces cerevisiae
-
-
1.1.1.42 peroxisome
-
 Aspergillus nidulans 5777
-
1.1.1.42 peroxisome IDP3 contains a canonical type I peroxisomal targeting sequence, a carboxyl-terminal Cys-Lys-Leu tripeptide. IDP3 is normally strictly peroxisomal Saccharomyces cerevisiae 5777
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.42 Mg2+ activates Saccharomyces cerevisiae
1.1.1.42 Mg2+ activates Aspergillus nidulans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.42 isocitrate + NADP+ Saccharomyces cerevisiae
-
 2-oxoglutarate + NADPH + H+ + CO2
-
 ?
1.1.1.42 isocitrate + NADP+ Aspergillus nidulans
-
 2-oxoglutarate + NADPH + H+ + CO2
-
 ?
1.1.1.42 isocitrate + NADP+ Saccharomyces cerevisiae MMY011
-
 2-oxoglutarate + NADPH + H+ + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.42 Aspergillus nidulans
-
-
-
1.1.1.42 Saccharomyces cerevisiae
-
-
-
1.1.1.42 Saccharomyces cerevisiae MMY011
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.42 recombinant His-tagged IDPA from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Aspergillus nidulans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.42 isocitrate + NADP+
-
 Saccharomyces cerevisiae 2-oxoglutarate + NADPH + H+ + CO2
-
 ?
1.1.1.42 isocitrate + NADP+
-
 Aspergillus nidulans 2-oxoglutarate + NADPH + H+ + CO2
-
 ?
1.1.1.42 isocitrate + NADP+
-
 Saccharomyces cerevisiae MMY011 2-oxoglutarate + NADPH + H+ + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.42 IDP3
-
 Saccharomyces cerevisiae
1.1.1.42 IDPA
-
 Aspergillus nidulans
1.1.1.42 NADP+-specific isocitrate dehydrogenase
-
 Saccharomyces cerevisiae
1.1.1.42 NADP+-specific isocitrate dehydrogenase
-
 Aspergillus nidulans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.42 7.5
-
 IDP2 Saccharomyces cerevisiae
1.1.1.42 8
-
-
 Aspergillus nidulans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.42 5.5 9 80% of maximal activity in the pH range of pH 7.0-9.0 Aspergillus nidulans

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.42 NADP+ specific for Saccharomyces cerevisiae
1.1.1.42 NADP+ specific for Aspergillus nidulans

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.1.42 Saccharomyces cerevisiae IDP2, isoelectric focusing
-
 6.5
1.1.1.42 Aspergillus nidulans IDPA, isoelectric focusing
-
 7.4
1.1.1.42 Saccharomyces cerevisiae IDP1, isoelectric focusing
-
 8.2
1.1.1.42 Saccharomyces cerevisiae IDP3, isoelectric focusing
-
 8.9

General Information

EC Number General Information Comment Organism
1.1.1.42 physiological function IDP3 provides the NADPH required for beta-oxidation of some fatty acids in the peroxisome Saccharomyces cerevisiae