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Literature summary extracted from

  • Shin, S.W.; Kil, I.S.; Park, J.W.
    Cytosolic NADP(+)-dependent isocitrate dehydrogenase regulates cadmium-induced apoptosis (2010), Biochem. Pharmacol., 79, 1072-1080.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.42 expression of His-tagged mutant enzymes C269S and C379S in Escherichia coli Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.42 C269S site-directed mutagenesis, is not sensitive to inhibition by Cd2+ in contrast to the wild-type enzyme Homo sapiens
1.1.1.42 C379S site-directed mutagenesis, is inhibited by Cd2+ in a similar manner to the wild-type Homo sapiens
1.1.1.42 additional information transfection of HEK-293 cells with an IDPc small interfering RNA significantly decreases the activity of IDPc and enhances cellular susceptibility to cadmium-induced apoptosis as indicated by the morphological evidence of apoptosis, DNA fragmentation and condensation, cellular redox status, mitochondria redox status and function, and the modulation of apoptotic marker proteins, overview. DNA fragmentation is enhanced in IDPc siRNA-transfected HEK293 cells compared to control cells upon exposure to cadmium Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.42 Cd2+ inhibits the purified enzyme and the enzyme in cells, NADP+ does not protect. No inhibition of IDPc mutant S269S. DNA fragmentation is enhanced in IDPc siRNA-transfected HEK293 cells compared to control cells upon exposure to cadmium Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.42 cytosol cytosolic isozyme IDPc, subcellular localization study, overview Homo sapiens 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.42 Mg2+ activates Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.42 isocitrate + NADP+ Homo sapiens
-
 2-oxoglutarate + NADPH + H+ + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.42 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.42 recombinant His-tagged mutant enzymes C269S and C379S from Escherichia coli by nickel affinity chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.42 HEK-293 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.42 isocitrate + NADP+
-
 Homo sapiens 2-oxoglutarate + NADPH + H+ + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.42 cytosolic NADP(+)-dependent isocitrate dehydrogenase
-
 Homo sapiens
1.1.1.42 IDPc
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.42 25
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.42 7.4
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.42 NADP+ dependent on Homo sapiens

General Information

EC Number General Information Comment Organism
1.1.1.42 additional information knockdown of IDPc expression in HEK293 cells greatly enhances apoptosis induced by cadmium. DNA fragmentation is enhanced in IDPc siRNA-transfected HEK293 cells compared to control cells upon exposure to cadmium Homo sapiens