EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.12.99.6 | membrane | - |
Hydrogenimonas thermophila | 16020 | - |
1.12.99.6 | periplasm | localized in both, the periplasmic space and membrane | Hydrogenimonas thermophila | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.12.99.6 | 55000 | - |
SDS-PAGE | Hydrogenimonas thermophila |
1.12.99.6 | 86300 | - |
gel filtration | Hydrogenimonas thermophila |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.12.99.6 | Hydrogenimonas thermophila | - |
- |
- |
1.12.99.6 | Hydrogenimonas thermophila EPI-55-1%T | - |
- |
- |
EC Number | Oxidation Stability | Organism |
---|---|---|
1.12.99.6 | the purified enzyme retains 37% of its initial activity after 72 h of air exposure. After 96 h of air exposure, no activity of the purified enzyme is detected | Hydrogenimonas thermophila |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.12.99.6 | Q-Sepharose column chromatography, Biogel hydroxyapatite column chromatography, and Superdex 200 gel filtration | Hydrogenimonas thermophila |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.12.99.6 | 2 | - |
crude extract, at 55°C, pH 7.0, using methylene blue as electron acceptor | Hydrogenimonas thermophila |
1.12.99.6 | 30 | - |
after 152fold purification, at 55°C, pH 7.0, using dichloroindophenol as electron acceptor | Hydrogenimonas thermophila |
1.12.99.6 | 55 | - |
after 152fold purification, at 55°C, pH 7.0, using methyl viologen as electron acceptor | Hydrogenimonas thermophila |
1.12.99.6 | 344 | - |
after 152fold purification, at 55°C, pH 7.0, using methylene blue as electron acceptor | Hydrogenimonas thermophila |
1.12.99.6 | 484 | - |
after 152fold purification, at 80°C, pH 7.0, using methylene blue as electron acceptor | Hydrogenimonas thermophila |
1.12.99.6 | 1078 | - |
after 152fold purification, at 55°C, pH 7.0, using phenazine methosulfate as electron acceptor | Hydrogenimonas thermophila |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.12.99.6 | H2 + methyl viologen | - |
Hydrogenimonas thermophila | H+ + reduced methyl viologen | - |
? | |
1.12.99.6 | H2 + methyl viologen | - |
Hydrogenimonas thermophila EPI-55-1%T | H+ + reduced methyl viologen | - |
? | |
1.12.99.6 | H2 + oxidized dichloroindophenol | - |
Hydrogenimonas thermophila | H+ + reduced dichloroindophenol | - |
? | |
1.12.99.6 | H2 + oxidized dichloroindophenol | - |
Hydrogenimonas thermophila EPI-55-1%T | H+ + reduced dichloroindophenol | - |
? | |
1.12.99.6 | H2 + oxidized methylene blue | - |
Hydrogenimonas thermophila | H+ + reduced methylene blue | - |
? | |
1.12.99.6 | H2 + oxidized methylene blue | - |
Hydrogenimonas thermophila EPI-55-1%T | H+ + reduced methylene blue | - |
? | |
1.12.99.6 | H2 + oxidized sodium hydrosulfite | - |
Hydrogenimonas thermophila | H+ + reduced sodium hydrosulfite | - |
? | |
1.12.99.6 | H2 + oxidized sodium hydrosulfite | - |
Hydrogenimonas thermophila EPI-55-1%T | H+ + reduced sodium hydrosulfite | - |
? | |
1.12.99.6 | H2 + phenazine methosulfate | best electron acceptor | Hydrogenimonas thermophila | H+ + reduced phenazine methosulfate | - |
? | |
1.12.99.6 | additional information | no activity is observed using NAD+, 2,3-dimethoxy methyl-1,4-benzoquinone, and potassium ferricyanide as electron acceptors | Hydrogenimonas thermophila | ? | - |
? | |
1.12.99.6 | additional information | no activity is observed using NAD+, 2,3-dimethoxy methyl-1,4-benzoquinone, and potassium ferricyanide as electron acceptors | Hydrogenimonas thermophila EPI-55-1%T | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.12.99.6 | heterodimer | - |
Hydrogenimonas thermophila |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.12.99.6 | [NiFe] hydrogenase | - |
Hydrogenimonas thermophila |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.12.99.6 | 80 | - |
at pH 6.0, using 10 mM methyl viologen or 20 mM sodium hydrosulfite as electron acceptors | Hydrogenimonas thermophila |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.12.99.6 | 10 | 90 | at 90°C the enzyme retains 83% of maximum activity, at lower temperatures hydrogenase activity remains the same as at 10°C (13% of maximum activity), similar activity is observed between 40 and 60°C (about 70% of maximum activity) | Hydrogenimonas thermophila |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.12.99.6 | 9 | - |
50 mM Tris-HCl | Hydrogenimonas thermophila |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.12.99.6 | 6 | 10.5 | at pH 9.5 activity decreases to 28% of activity at pH 9.0, hydrogenase activity decreases completely at pH 10.5, while at pH 6.0 45% of activity is retained | Hydrogenimonas thermophila |