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Literature summary extracted from

  • Andreou, A.; Goebel, C.; Hamberg, M.; Feussner, I.
    A bisallylic mini-lipoxygenase from cyanobacterium Cyanothece sp. that has an iron as cofactor (2010), J. Biol. Chem., 285, 14178-14186.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.33 expressed in Escherichia coli BL21 Star cells Crocosphaera subtropica ATCC 51142

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.33 Fe2+ LOX2 contains iron with an iron to protein ratio of 45% Crocosphaera subtropica ATCC 51142

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.33 65000
-
 SDS-PAGE Crocosphaera subtropica ATCC 51142

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.33 Crocosphaera subtropica ATCC 51142 B7JX99
-
-
1.13.11.33 Crocosphaera subtropica ATCC 51142 PCC 8801 B7JX99
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.33 HisTrap column chromatography, gel filtration Crocosphaera subtropica ATCC 51142

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.33 1,2-diarachidonoylglycerophosphocholine + O2
-
 Crocosphaera subtropica ATCC 51142 ?
-
 ?
1.13.11.33 1,2-diarachidonoylglycerophosphocholine + O2
-
 Crocosphaera subtropica ATCC 51142 PCC 8801 ?
-
 ?
1.13.11.33 arachidonic acid + O2
-
 Crocosphaera subtropica ATCC 51142 13-hydroperoxyeicosatetraenoic acid + (5Z,8Z,11R,12E,14Z)-11-hydroperoxyicosa-5,8,12,14-tetraenoic acid + (5Z,8E,11Z,13E,15S)-15-hydroperoxyicosa-5,8,11,13-tetraenoic acid
-
 ?
1.13.11.33 arachidonic acid + O2
-
 Crocosphaera subtropica ATCC 51142 PCC 8801 13-hydroperoxyeicosatetraenoic acid + (5Z,8Z,11R,12E,14Z)-11-hydroperoxyicosa-5,8,12,14-tetraenoic acid + (5Z,8E,11Z,13E,15S)-15-hydroperoxyicosa-5,8,11,13-tetraenoic acid
-
 ?
1.13.11.33 linoleate + O2
-
 Crocosphaera subtropica ATCC 51142 (9Z,11R,12Z)-11-hydroperoxyoctadeca-9,12-dienoate + (9Z,11E,13S)-13-hydroperoxyoctadeca-9,11-dienoate + (9R,10E,12Z)-9-hydroperoxyoctadeca-10,12-dienoate (9Z,11R,12Z)-11-hydroperoxyoctadeca-9,12-dienoate is the primary product ?
1.13.11.33 linoleate + O2
-
 Crocosphaera subtropica ATCC 51142 PCC 8801 (9Z,11R,12Z)-11-hydroperoxyoctadeca-9,12-dienoate + (9Z,11E,13S)-13-hydroperoxyoctadeca-9,11-dienoate + (9R,10E,12Z)-9-hydroperoxyoctadeca-10,12-dienoate (9Z,11R,12Z)-11-hydroperoxyoctadeca-9,12-dienoate is the primary product ?
1.13.11.33 linolenate + O2
-
 Crocosphaera subtropica ATCC 51142 11-hydroperoxyoctadecatrienoic acid + 9-hydroperoxyoctadecatrienoic acid + 13-hydroperoxyoctadecatrienoic acid
-
 ?
1.13.11.33 linolenate + O2
-
 Crocosphaera subtropica ATCC 51142 PCC 8801 11-hydroperoxyoctadecatrienoic acid + 9-hydroperoxyoctadecatrienoic acid + 13-hydroperoxyoctadecatrienoic acid
-
 ?
1.13.11.33 additional information incubation of the enzyme with [(11S)-2H]-linoleic acid leads to the formation of hydroperoxides that have lost the deuterium label, thus suggesting that LOX2 catalyzes antarafacial oxygenation as opposed to the mechanism of manganese lipoxygenase Crocosphaera subtropica ATCC 51142 ?
-
 ?
1.13.11.33 additional information incubation of the enzyme with [(11S)-2H]-linoleic acid leads to the formation of hydroperoxides that have lost the deuterium label, thus suggesting that LOX2 catalyzes antarafacial oxygenation as opposed to the mechanism of manganese lipoxygenase Crocosphaera subtropica ATCC 51142 PCC 8801 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.33 LOX2
-
 Crocosphaera subtropica ATCC 51142

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.33 9.3
-
-
 Crocosphaera subtropica ATCC 51142

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.13.11.33 6 10
-
 Crocosphaera subtropica ATCC 51142